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F. C. Abib, A. R. C. Schaefer, Tâ. M. C. Schaefer, R. Godois, R. A. Laing; Corneal Endothelial Cell Sample Profile on Specular Microscopy With Reliability and Reproducibility. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4286.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the corneal endothelial sample profile in specular microscopy with reliability and reproducibility.
A retrospective study was performed of 100 right eyes of patients who had undergone corneal specular microscopy, with different clinical situations, in March 2006 in a private clinic. The Bio-Optics specular microscopy was used with the Bambi 2000 Plus software. To compute the sample size we used the software package, CELLS ANALYZER - Corneal Endothelial Statistical LabPat Rec with planned reliability degree (RD) 95%. The sampling process of acquiring endothelial images and counting those cell images were stoped when the calculated relative error (RE) < 0.05. Endothelial samples were evaluated in relation to calculated sample size, considered number of endothelial images, and cell number of the specular microscopy exams.
The sex distribution was 64 (64%) of the eyes of female patients and 36(36%) of male patients. The minimum age was 16 years, the maximum 96 years and the average 51±19 years. The smallest computed sample size was 57 cells, the largest 315 cells and the average was 138±45 cells; The smallest endothelial sampling had 1 image, the largest 7 images and the average 2.35±1.05 images.
To make corneal specular microscopy with reliability and reproducibility (RD 95% and RE<0.05) the sampling process needed 1 to 7 (2.35±1) different images and a large number of endothelial cells counted in different areas of corneal endothelial mosaics.
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