May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
LPS - Accentuated Inflammation and Apoptosis in Corneal Injury Models
Author Affiliations & Notes
  • H. Liang
    Ophthalmology-Hosp Paris, CHNO des Quinze-Vights, Paris, France
    Inserm U598, Cordeliers Biomedical Institute, Paris, France
  • F. Brignole-Baudouin
    Inserm U598, Cordeliers Biomedical Institute, Paris, France
    Department of Toxicology, Faculty of Biological and Pharmacological Sciences, Paris, France
  • A. Labbé
    Ophthalmology-Hosp Paris, CHNO des Quinze-Vights, Paris, France
    Inserm U598, Cordeliers Biomedical Institute, Paris, France
  • A. Pauly
    Inserm U598, Cordeliers Biomedical Institute, Paris, France
  • J.-M. Warnet
    Department of Toxicology, Faculty of Biological and Pharmacological Sciences, Paris, France
  • C. Baudouin
    Ophthalmology-Hosp Paris, CHNO des Quinze-Vights, Paris, France
    Inserm U598, Cordeliers Biomedical Institute, Paris, France
  • Footnotes
    Commercial Relationships H. Liang, None; F. Brignole-Baudouin, None; A. Labbé, None; A. Pauly, None; J. Warnet, None; C. Baudouin, None.
  • Footnotes
    Support INSERM U598 and Quinze-Vingts National Ophthalmology Hospital
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4300. doi:
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      H. Liang, F. Brignole-Baudouin, A. Labbé, A. Pauly, J.-M. Warnet, C. Baudouin; LPS - Accentuated Inflammation and Apoptosis in Corneal Injury Models. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4300.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To compare and understand the inflammatory and apoptotic effects of LPS instillations in three rabbit cornea injury models using a new in vivo confocal microscope (IVCM) and immunohistology.

Methods:: Adult male New Zealand albino rabbits were used in this study. Three corneal models were tested: corneal incision; corneal epithelium scraping and corneal suture. Eight rabbits were used in each model, and these three groups were subdivided into two groups, with or without LPS instillation (saline as control) during 8 days. Rabbit corneas were analyzed in vivo by using the Rostock Cornea Module (RCM) of the Heidelberg Retina Tomograph (HRT)-II. The immunohistology was used to evaluate the inflammatory, proliferating and apoptotic cells following the different injuries.

Results:: The RCM/HRT successfully presented high-quality images allowing analysis of all pathological corneal layers. Compared to saline-receiving groups, LPS provoked earlier appearance of corneal surface conjunctivalization and stromal neovascularization, induced more inflammatory infiltration in the cornea stroma close to the lesions, and earlier infiltration of dendritic-like cells in the basal epithelial cell layer. Immunohistology was correlated with in vivo findings and confirmed these results by showing more important infiltration of ICAM-1+, RLA-DR+, CD11b+, CD11c+, TNF-α+, TNFR1+, and TLR4+ inflammatory cells, KI 67+ proliferating cells and TUNEL+ apoptotic cells in the cornea.

Conclusions:: The results indicate that during corneal injuries, LPS is a potent inflammatory stimulus and its exposure has major effects on determinants of inflammation, angiogenesis, conjunctivalization and apoptosis.

Keywords: inflammation • neovascularization • wound healing 
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