May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Low Dose -Irradiation as a Theurapeutic Approach of Glaucoma in DBA/2J Mice
Author Affiliations & Notes
  • G. Labunskay
    University of Nebraska Medical Center, Omaha, Nebraska
    Pharmacology and Experimental Neuroscience,
  • L. Camras
    University of Nebraska Medical Center, Omaha, Nebraska
    Pharmacology and Experimental Neuroscience,
  • G. Zhan
    University of Nebraska Medical Center, Omaha, Nebraska
    Ophthalmology and Visual Sciences,
  • C. B. Camras
    University of Nebraska Medical Center, Omaha, Nebraska
    Ophthalmology and Visual Sciences,
  • J. Kipnis
    University of Nebraska Medical Center, Omaha, Nebraska
    Pharmacology and Experimental Neuroscience,
    Ophthalmology and Visual Sciences,
  • Footnotes
    Commercial Relationships G. Labunskay, None; L. Camras, None; G. Zhan, None; C.B. Camras, None; J. Kipnis, None.
  • Footnotes
    Support Nebraska Tabacco Settlement Fund
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4373. doi:
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    • Get Citation

      G. Labunskay, L. Camras, G. Zhan, C. B. Camras, J. Kipnis; Low Dose -Irradiation as a Theurapeutic Approach of Glaucoma in DBA/2J Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4373.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To determine if low dose whole body γ-irradiation is neuroprotectivefor DBA/2J mice, which inherit glaucoma along with increasedintraocular pressure (IOP).

 
Methods:
 

DBA/2J mice received either a sham procedure, or a single irradiationof 300 rad at the age of 9 months (onset of the disease) or12 months (peak of the disease). The survival of retinal ganglioncells (RGCs) was assessed 3 months later and compared with theage matched control group. IOP was measured using a pneumatonometeron a monthly basis for 3 months, beginning immediately afterthe irradiation.

 
Results:
 

Low-dose γ-irradiation led to a reduction of IOP in both treatedgroups of mice, beginning 1-2 months after irradiation (Table1). Along with reduced IOP, irradiated animals showed a significantincrease in RGC survival in both groups (Table 2). RGC survivalwas accompanied by significant upregulation of Muller glialcells for irradiated mice (p =0.009, n=7 at 9 months & p=0.035,n=10 at 12 months) that may implicate a possible mechanism forneuroprotection.Table1Table2 

 

 
Conclusions:
 

Low dose whole body γ-irradiation-induced neuroprotection, accompaniedby IOP reduction and activation of Muller cells in the retinafor DBA/J2 mice, may have significant implications for the developmentof new therapeutic approaches for glaucoma.

 
Keywords: neuroprotection • intraocular pressure • radiation therapy 
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