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G. Labunskay, L. Camras, G. Zhan, C. B. Camras, J. Kipnis; Low Dose -Irradiation as a Theurapeutic Approach of Glaucoma in DBA/2J Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4373.
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To determine if low dose whole body γ-irradiation is neuroprotectivefor DBA/2J mice, which inherit glaucoma along with increasedintraocular pressure (IOP).
DBA/2J mice received either a sham procedure, or a single irradiationof 300 rad at the age of 9 months (onset of the disease) or12 months (peak of the disease). The survival of retinal ganglioncells (RGCs) was assessed 3 months later and compared with theage matched control group. IOP was measured using a pneumatonometeron a monthly basis for 3 months, beginning immediately afterthe irradiation.
Low-dose γ-irradiation led to a reduction of IOP in both treatedgroups of mice, beginning 1-2 months after irradiation (Table1). Along with reduced IOP, irradiated animals showed a significantincrease in RGC survival in both groups (Table 2). RGC survivalwas accompanied by significant upregulation of Muller glialcells for irradiated mice (p =0.009, n=7 at 9 months & p=0.035,n=10 at 12 months) that may implicate a possible mechanism forneuroprotection.Table1Table2
Low dose whole body γ-irradiation-induced neuroprotection, accompaniedby IOP reduction and activation of Muller cells in the retinafor DBA/J2 mice, may have significant implications for the developmentof new therapeutic approaches for glaucoma.
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