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H. Tomita, E. Sugano, H. Yawo, T. Ishizuka, H. Isago, S. Narikawa, M. Yoshioka, M. Tamai; Differences of Visually Evoked Potentials Between in Young and Aged RCS Rats Transferred Channelopsin 2 Gene. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4514.
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The channelopsin-2 (chop2) gene was transferred to young and aged Royal Colleage Surgeons (RCS) and differences of recovered visually evoked potentials (VEPs) were investigated.
Male six (young) and 10 month old (aged) Royal College of Surgeons (RCS: rdy/rdy) rats were used in this study. The N-terminal fragment 1-315 of channelopsin-2 was fused to a fluorescent protein, venus, in frame at the end of the chop2 coding fragment. The viral vector construct (AAV-Chop2V) for the expression of the Chop2V in the retina was made by subcloning into an adeno-associated virus vector cassette including a CAG promoter. The AAV-Chop2V virus vector was injected into vitreous cavity in left eyes of RCS rats. As a control, AAV-EGFP virus vector was applied for right eyes. The VEP recordings were performed at pre-injection or once a week after the virus vector injection.
The VEPs in young and aged RCS rat’s eyes with applied AAV-EGFP were not observed. In young and aged rats injected AAV-Chop2V the VEPs were observed 2 weeks after the injection. However the waveforms obtained from young RCS rats were different from those of old RCS rats. The latency of young RCS rats were significantly earlier than those of old rats but no difference in amplitudes between them. The expression of Chop2V in the retina of both rats was almost equally observed in inner retinal neurons including ganglion cells.
Advanced photoreceptor degeneration in RCS rats may affect to the inner retinal viability and their function recovered with this new method.
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