May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Evaluation of the RPS Adeno Detector for Detecting Adenovirus From Ocular Specimens in the Laboratory Setting
Author Affiliations & Notes
  • N. M. Siamak
    UPMC Eye Center, Department of Ophthalmology, Pittsburgh, Pennsylvania
    Department of Ophthalmology, University of Pittsburgh School of Medicine, The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Science Research Center, Eye and Ear Institute, Pittsburgh, Pennsylvania
  • R. P. Kowalski
    Department of Ophthalmology, University of Pittsburgh School of Medicine, The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Science Research Center, Eye and Ear Institute, Pittsburgh, Pennsylvania
  • P. P. Thompson
    Department of Ophthalmology, University of Pittsburgh School of Medicine, The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Science Research Center, Eye and Ear Institute, Pittsburgh, Pennsylvania
  • E. G. Romanowski
    Department of Ophthalmology, University of Pittsburgh School of Medicine, The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Science Research Center, Eye and Ear Institute, Pittsburgh, Pennsylvania
  • R. M. Q. Shanks
    Department of Ophthalmology, University of Pittsburgh School of Medicine, The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Science Research Center, Eye and Ear Institute, Pittsburgh, Pennsylvania
  • Y. J. Gordon
    Department of Ophthalmology, University of Pittsburgh School of Medicine, The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Science Research Center, Eye and Ear Institute, Pittsburgh, Pennsylvania
  • Footnotes
    Commercial Relationships N.M. Siamak, None; R.P. Kowalski, None; P.P. Thompson, None; E.G. Romanowski, None; R.M.Q. Shanks, None; Y.J. Gordon, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4731. doi:
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      N. M. Siamak, R. P. Kowalski, P. P. Thompson, E. G. Romanowski, R. M. Q. Shanks, Y. J. Gordon; Evaluation of the RPS Adeno Detector for Detecting Adenovirus From Ocular Specimens in the Laboratory Setting. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4731.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: The RPS Adeno Detector (South Williamsport, PA) has been FDA approved for directly detecting adenovirus from the conjunctiva of patients in the office setting. In this retrospective study, we evaluated the RPS Adeno Detector for detecting adenovirus from ocular swabs placed in chlamydia/viral transport medium that were submitted to the laboratory.

Methods:: The RPS Adeno Detector was evaluated for laboratory use against: 1) 11 archived true-positive clinical specimens that were cell-culture positive, shell vial positive, and PCR positive for adenovirus, 2) 16 true-negative specimens (4 archived clinical specimens that were culture cell positive for HSV, 4 archived clinical isolates each of Haemophilus influenzae, Streptococcus pneumoniae, and Staphylococcus aureus.), and 3) positive and negative controls. The RPS Adeno Detector was modified to test 0.1 ml of study specimens in chlamydia/viral transport medium (Bartels, Jamestown, NY). Testing was interpreted in a masked study with 5 observers at time-points of 10 and 30 minutes. The main outcome measures were: sensitivity, specificity, positive predictive value, negative predictive value, and efficiency.

Results:: The outcome measures of the RPS Adeno Detector for detecting adenovirus in a laboratory setting were: 9% sensitivity, 100% specificity, 100% positive predictive value, 61.5 % negative predictive value, and 62.9% efficiency.

Conclusions:: Under the conditions tested, the RPS Adeno Detector was not reliable for detecting adenovirus from ocular specimens submitted to the laboratory in chlamydia/viral transport medium. Further refinements will be necessary to improve the present RPS Adeno Detector for future laboratory testing of submitted samples.

Keywords: adenovirus • detection • conjunctivitis 
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