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K. Aoki, X.-H. Jin, T. Ohguchi, Y. Tagawa, S. Ohno; Molecular Epidemiology of Epidemic Keratoconjunctivitis Due to Human Adenovirus 8 and 19 in Japan. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4732.
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© ARVO (1962-2015); The Authors (2016-present)
To detect the genetic changes in adenovirus serotypes 8 and 19(HAdV-8 and HAdV-19) isolated in Japan in 2003, by DNA restriction analysis and DNA sequence analysis of the full-length hexon and fiber genes.
Two HAdV-8 and three HAdV-19 strains were isolated from the patients with epidemic keratoconjunctivitis in Japan between July and September 2003 and identified as HAdV-8 or HAdV-19 by a neutralization test with serotype-specific antiserum. Viral DNA was extracted and then DNA restriction analysis was performed. The entirety of each hexon gene and fiber gene was amplified by PCR method, and then the nucleotide sequences of the entire hexon and fiber genes were determined.
Two HAdV-8 strains exhibited completely different restriction patterns from those of HAdV-8 prototype. One of them exhibited identical restriction patterns to those of our previously reported HAdV-8 Kobe strain, and the other exhibited different restriction pattern from HAdV-8 Kobe strain only under HindIII enzyme digestion. The hexon sequence of both HAdV-8 strains was identical to that of HAdV-8 Kobe strain, whereas in the fiber sequence, they showed three and four point mutations respectively, comparing to HAdV-8 Kobe strain. Three HAdV-19 strains exhibited different restriction patterns from each other and from those of Ad19 prototype and all previously known HAdV-19 genome types. In the hexon sequence, one HAdV-19 strain showed one point mutation and the other two strains showed the same two point mutations comparing to HAdV-19a. All the HAdV-19 strains showed the same fiber sequence as that of HAdV-19a.
Gene mutations occur frequently in both Had-8 and HAdV-19.
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