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P. A. Moore, S. D. Boveland, J. Mysore, U. Dietrich, C. Jarret, K. P. Carmichael; Immunohistochemical Identification of IL-1B in Equine Fungal Keratitis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4736.
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Identify the role of IL-1B in the pathogenesis of equine fungal keratitis and purulonecrotic keratitis
Paraffin-embedded normal (n=3), purulonecrotic (n=10) and fungal keratitis (n=9) corneal samples were evaluated immunohistochemically for IL-IB. The number of immunoreactive inflammatory cells (macrophages and neutrophils) was counted in 10 high power fields and the staining intensity (SI) scored from 1-4. A student’s t-test was performed to compare the number of immunoreactive inflammatory cells (IC) and SI between the all three groups. Significance was set at p≤ 0.05.
The mean IC cout for the three groups were 2.86 for the purulonecrotic, 4.67 for the fungal keratitis and 0 for the control samples. The mean SI for the three groups were 2.10 for the purulonecrotic, 2.11 for the fungal keratitis and 0 for the control samples. The number of IC, and SI was significantly higher for IL-1B in the purulonecrotic and fungal keratitis samples when compared to the controls. Three was no significant difference in the number of IC and SI between purulonecrotic and fungal keratitis samples.
IL-1B is expressed in equine purulonecrotic and fungal keratitis, and may play are role in their pathogenesis.
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