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I. L. Romero, C. Barreto, L. M. J. Mimica, R. Y. Hida; Antimicrobial Influence of Polymerization Process and Degradation Products of Ethyl-cyanoacrylate in vitro for Microorganisms Related to Corneal Infection. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4748.
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to analyze the antibacterial effect in vitro of the ethyl-cyanoacrylate in different moments of the polymerization process and the influence of its degradation products for some microorganisms related to corneal infections.
We analyzed the following microorganisms: Pseudomonas aeruginosa (ATCC27853), Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923), and Klebsiella pneumoniae (ATCC13883) using Müller-Hinton culture broth and Streptococcus pneumoniae (ATCC49619) using blood agar broth. The same volume of ethyl-cyanoacrylate was dropped directly to blank sterile 6 mm disks, which were placed in the plates with respective microorganisms. The corresponding inhibition halo was then measured in millimeters in different moments of the polymerization process (T0, T10, T30, T45, T60 and T120 minutes). Blank disk with polymerized adhesive with water (TH2O) was analyzed to study the degradation products. The plates were incubated at 35±2°C and their inhibition halos were measured after 24 hours.
There was no antibacterial effect against Pseudomonas aeruginosa in all moments.Inhibition halos were observed for Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Streptococcus pneumoniae. Furthermore, T0 had significant inhibition halo compared to all other moments for the microorganisms above (p<0.05). Inhibition halo for TH2O was observed for all microorganisms above. Degradation products had an important role on the antimicrobial effect for Klebsiella pneumoniae.
Polymerization process and the degradation products have an important role on the antimicrobial effect, in vitro for microorganisms related to corneal infection, except for Pseudomonas aeruginosa.
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