May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
TGF-Beta Induces Osteopontin in Uveal Melanoma Cells
A. H. Wolf; I. W. Reiniger; U. C. Schaller; D. Kook; A. J. Mueller; A. Kampik; U. C. Welge-Luessen
Author Affiliations & Notes
  • A. H. Wolf
    Dept of Ophthalmology, University of Munich LMU, Munich, Germany
  • I. W. Reiniger
    Dept of Ophthalmology, University of Munich LMU, Munich, Germany
  • U. C. Schaller
    Dept of Ophthalmology, University of Munich LMU, Munich, Germany
  • D. Kook
    Dept of Ophthalmology, University of Munich LMU, Munich, Germany
  • A. J. Mueller
    Dept of Ophthalmology, Klinikum Augsburg, Augsburg, Germany
  • A. Kampik
    Dept of Ophthalmology, University of Munich LMU, Munich, Germany
  • U. C. Welge-Luessen
    Dept of Ophthalmology, University of Munich LMU, Munich, Germany
  • Footnotes
    Commercial Relationships A.H. Wolf, None; I.W. Reiniger, None; U.C. Schaller, None; D. Kook, None; A.J. Mueller, None; A. Kampik, None; U.C. Welge-Luessen, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4760. doi:
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      A. H. Wolf, I. W. Reiniger, U. C. Schaller, D. Kook, A. J. Mueller, A. Kampik, U. C. Welge-Luessen; TGF-Beta Induces Osteopontin in Uveal Melanoma Cells. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4760.

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Abstract

Purpose:: TGF-beta has been shown to have a tumor promoting effect in uveal melanoma. In carcinomas TGF-beta is thought to promote the ability to metastasize. Osteopontin (OPN) is a promising tumor marker for uveal melanoma and other malignancies. Aim of our study was to investigate the coherence of the TGF-beta and the OPN in uveal melanoma cells.

Methods:: Cells of 5 untreated uveal melanoma were grown until confluency. For specification of cells, immunohistological staining against S100, HMB-45 and OPN was performed. Confluent cells were then treated with TGF-beta at 1,0 mg/ml for 6 to 48 hrs. Concentrations of OPN were measured thereafter by quantititive RT-PCR.

Results:: All grown cells showed positive staining for S 100, HMB-45 and OPN. Concentrations of OPN increased markedly in 4 out of 5 uveal melanomas when treated with TGF-beta. Highest increase of OPN was found in cells treated for 24 hrs at 1mg/ml TGF-beta. At this concentration we found an up to 3-fold increase of OPN.

Conclusions:: TGF-beta induces OPN in uveal melanoma cells. Thus, elevated levels of OPN as found in patients with metastastatic uveal melanoma might be induced by increased TGF-beta. Our results point out the need to further investigate the role of TGF-beta in the genesis of uveal melanoma.

Keywords: melanoma • tumors • pathology: human 
© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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