May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Repression of Genes Associated With Apoptosis, Development and Pigmentation in Uveal Melanoma
Author Affiliations & Notes
  • S. Landreville
    Ophtalmologie, Université Laval/CRCHUL, Ste-Foy, Quebec, Canada
  • V. Boily
    Ophtalmologie, Université Laval/CRCHUL, Ste-Foy, Quebec, Canada
  • S. Champagne
    Ophtalmologie, Université Laval/CRCHUL, Ste-Foy, Quebec, Canada
  • Québec Uveal Melanoma Group
    Ophtalmologie, Université Laval/CRCHUL, Ste-Foy, Quebec, Canada
  • A. Rousseau
    Ophtalmologie, Université Laval/CRCHUL, Ste-Foy, Quebec, Canada
  • C. Salesse
    Ophtalmologie, Université Laval/CRCHUL, Ste-Foy, Quebec, Canada
  • Footnotes
    Commercial Relationships S. Landreville, None; V. Boily, None; S. Champagne, None; A. Rousseau, None; C. Salesse, None.
  • Footnotes
    Support CNIB, FRSQ, NSERC
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4775. doi:
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      S. Landreville, V. Boily, S. Champagne, Québec Uveal Melanoma Group, A. Rousseau, C. Salesse; Repression of Genes Associated With Apoptosis, Development and Pigmentation in Uveal Melanoma. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4775.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: There is increasing evidence that cancer can arise from a cancer stem cell, a tumor-initiating cell that has properties similar to those of stem cells. We demonstrated previously that uveal melanoma cells contain a small subpopulation of cells that exhibit a cancer stem cell-like phenotype. Subtractive library was thus prepared to identify genes which are down-regulated in the primary tumor when compared to the normal tissue.

Methods:: Suppressive Subtractive Hybridization (SSH) was used to isolate genes that are repressed in uncultured primary tumors of uveal melanoma compared to normal uveal melanocytes. A library was thus constructed from the resulting subtracted cDNAs. Genes associated with apoptosis, development and pigmentation were then confirmed to be down- regulated in uncultured primary tumors using semi-quantitative RT-PCR. We also studied the methylation status of some of these genes by performing methylation-specific polymerase chain reaction (MSP-PCR).

Results:: The SSH library allowed to identify 112 genes repressed in primary uveal melanoma tumors. Semi-quantitative RT-PCR analyses confirmed the repression of selected genes implicated in apoptosis, development and pigmentation. MSP-PCR demonstrated hypermethylation of the promoter of some of these genes.

Conclusions:: The analysis of the subtracted library revealed genes that are repressed in primary tumor of uveal melanoma. Some of these genes are associated with apoptosis, development and pigmentation and thus provided additional evidence that uveal melanoma can arise from a cancer stem cell.

Keywords: tumors • candidate gene analysis • gene/expression 
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