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B. Burgess, T. A. Young, J. N. Moral, N. P. Rao, B. J. Glasgow, B. R. Straatsma; Fine Needle Aspiration Biopsy as a Source of Material in Choroidal Melanoma Cytogenetic Studies. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4777.
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To investigate the utility of intra-operative fine needle aspiration biopsy (FNAB) at the time of Iodine-125 plaque placement for choroidal melanoma as a source of material for histopathologic and cytogenetic studies.
Twenty-one consecutive eyes (21 patients) with a clinical diagnosis of choroidal melanoma had intra-operative transscleral FNAB prior to plaque placement. In each eye, a mean of 5 (range 3 to 7) samples were obtained via a 30 gauge needle and immediately processed. Biopsy material was allocated for (1) histopathologic processing and intra-operative diagnosis, (2) monosomy 3 testing, (3) cell culture for propagation, and (4) DNA and RNA analyses.
In 21 eyes, adequate material for a pathologic diagnosis of choroidal melanoma was obtained in 16 (76%) of the cases. Cytogenetic testing was possible in 15 (71%) of the cases and a finding of monosomy 3 occurred in 3 (20%) of the cases scored. Viable cell culture was achieved in 12 (57%) of the cases. Genomic DNA and total RNA were recovered in 13 (62%) of the cases with yields ranging from 720 ng to 3340 ng and 335 ng to 1750 ng, respectively.
FNAB at the time of plaque placement may provide sufficient material for histopathologic diagnosis, cytogenetic testing, cell culture and nucleic acid studies.
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