Purchase this article with an account.
C. Martins, E. Antecka, S. Di Cesare, C. Edelstein, D. Odashiro, M. N. Burnier, Jr.; Expression of KISS1 in Uveal Melanoma and Its Relationship With Prognosis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4779.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
KISS1, a human metastasis suppressor gene, is a well-established prognostic marker in skin melanoma, where its reduced expression is correlated with a high metastatic potential. Uveal Melanoma (UM) is the most common malignant intra-ocular tumor in adults. Despite the high accuracy of clinical diagnosis and advances in local treatment, approximately 50% of UM patients develop metastasis within ten years of initial diagnosis. The purpose of this study is to investigate the expression of KISS1 in UM and its potential value as a prognostic marker.
Thirty-seven cases of paraffin-embedded human uveal melanoma (UM) were immuno-stained with a KISS1 polyclonal antibody using the Ventana BenchMark fully automated machine. The immuno-staining was evaluated in a semi-quantitative method based on the extent and intensity of staining by double blind analysis. Clinical-pathological data were obtained, including age, gender, tumor cell type, and largest tumor dimension. The relationship between the clinical-pathological data and the expression of KISS1 was evaluated. Moreover, the survival rates of the patients were also assessed using a Kaplan-Meier test. Five UM cell lines (92.1, OCM-1, MKTBR, UW1 and SP6.5) were analyzed at the protein expression level, by immunohistochemical staining of cytospins and Western blot. In addition, quantitative real-time PCR was used to determine mRNA transcript levels of KISS1 and its receptor GPR54 in these cell lines.
The immunostainning of KISS1 displayed cytoplasmic staining in 84% of UM. A decrease in KISS1 expression was associated with higher risk of metastatic disease (P<0.05). Furthermore, we found that the expression of KISS1 and its receptor GPR54 were present in all 5 UM cells lines to varying degrees. Quantitative real-time PCR analysis confirmed the presence of KISS1 mRNA expression and its receptor GPR54 in all 5 human UM cell lines .
To the best of our knowledge this is the first time that KISS1 has been characterized in uveal melanoma. The correlation between KISS1 expression and uveal melanoma survival rate suggests an important role for KISS1 as a prognostic marker in this particular tumor.
This PDF is available to Subscribers Only