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S. Lofgren, R. M. Fernando, Y.-S. Ho, M. F. Lou; Reactivity and Repair in Thioltransferase (glutaredoxin) Knockout Lens Epithelial Cells After Oxidative Stress From UV Radiation or Peroxide Treatment. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4913.
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To investigate the response of UV radiation or peroxide in thioltransferase knockout lens epithelial cells. Thioltransferase is a repair enzyme involved in the regeneration of oxidized proteins.
Primary lens epithelial cells from wild type and thioltransferase knockout mice were exposed to either hydrogen peroxide or UV radiation. The GSH/GSSG ratio and activities of glutathione peroxidase and glutathione reductase were determined spectrophotometrically.
Both enzyme activities and the GSH/GSSG ratio immediately decreased after UVR exposure and peroxide treatment. All parameters recovered dose/concentration-dependently towards baseline within one hour, with faster recovery in the wild type cells.
The glutathione system reacts similarly to both UV radiation and peroxide oxidative stress. Thioltransferase plays an important role in the repair of oxidative stress to the glutathione system.
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