May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Identification of a New Angiogenesis Associated Gene in the Retina
Author Affiliations & Notes
  • E. P. Rakoczy
    Centre for Ophthalmology & Visual Science, University of Western Australia, Perth, Australia
    Department of Molecular Ophthalmology, Lions Eye Institute, Perth, Australia
  • J. Eade
    Centre for Ophthalmology & Visual Science, University of Western Australia, Perth, Australia
    Department of Molecular Ophthalmology, Lions Eye Institute, Perth, Australia
  • N. Binz
    Centre for Ophthalmology & Visual Science, University of Western Australia, Perth, Australia
    Department of Molecular Ophthalmology, Lions Eye Institute, Perth, Australia
  • J. Mills
    Centre for Ophthalmology & Visual Science, University of Western Australia, Perth, Australia
    Department of Molecular Ophthalmology, Lions Eye Institute, Perth, Australia
  • K. Simpson
    Division of Genetics and Bioinformatics, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
  • T. Speed
    Division of Genetics and Bioinformatics, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
  • C.-M. Lai
    Centre for Ophthalmology & Visual Science, University of Western Australia, Perth, Australia
    Department of Molecular Ophthalmology, Lions Eye Institute, Perth, Australia
  • C. Graham
    Centre for Ophthalmology & Visual Science, University of Western Australia, Perth, Australia
    Department of Molecular Ophthalmology, Lions Eye Institute, Perth, Australia
  • Footnotes
    Commercial Relationships E.P. Rakoczy, None; J. Eade, None; N. Binz, None; J. Mills, None; K. Simpson, None; T. Speed, None; C. Lai, None; C. Graham, None.
  • Footnotes
    Support Brian King Fellowship
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4964. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      E. P. Rakoczy, J. Eade, N. Binz, J. Mills, K. Simpson, T. Speed, C.-M. Lai, C. Graham; Identification of a New Angiogenesis Associated Gene in the Retina. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4964.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: We sought to identify genetic changes in the retinae of trVEGF029, an animal model that displays pathological changes consistent with non-proliferative and early stages of proliferative diabetic retinopathy.

Methods:: We used microarray to compare the genetic profile of the posterior eye cups of trVEGF029 to wild-type mice. Real-time PCR was used to confirm the changes in gene expression of a previously uncharacterised gene, NM_177853. The expression was studied between 1.5 and 15 weeks post-natal age of trVEGF029 and wild-type eyes. Multiple tissue expression was also identified by qPCR and immunohistochemistry of eye sections.

Results:: We identified a previously uncharacterised gene (NM_177853) upregulated in the posterior eye cups of trVEGF029. NM_177853 expression was shown to increase with age and development of retinal vascular changes, and is upregulated following mVEGF stimulation and hyperglycemia. It is expressed primarily in the retinal pigment epithelium and choroid of the eye, and in areas of the brain, kidney, ovary and embryo.

Conclusions:: Here we report the first analysis of NM_177853 a new gene associated with VEGF-induced retinal neovascularisation.

Keywords: retina • retinal neovascularization • diabetic retinopathy 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×