May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Glycocalyx Perturbation Seen in Retinal and Choroidal Microvessels of Streptozocin Induced Diabetic or Spontaneously Hypertensive Rats With Special Reference to Leukocyte-Endothelial Cell Adhesion
Author Affiliations & Notes
  • F. Kumase
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Ophthalmology,
  • S. Mohri
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Cardiovascular Physiology,
  • Y. Morizane
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Ophthalmology,
  • I. Takasu
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Ophthalmology,
  • H. Ohtsuki
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Ophthalmology,
  • A. Ohtsuka
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Human Morphology,
  • Footnotes
    Commercial Relationships F. Kumase, None; S. Mohri, None; Y. Morizane, None; I. Takasu, None; H. Ohtsuki, None; A. Ohtsuka, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4980. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      F. Kumase, S. Mohri, Y. Morizane, I. Takasu, H. Ohtsuki, A. Ohtsuka; Glycocalyx Perturbation Seen in Retinal and Choroidal Microvessels of Streptozocin Induced Diabetic or Spontaneously Hypertensive Rats With Special Reference to Leukocyte-Endothelial Cell Adhesion. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4980.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: The glycocalyx provides the cell surface with a negatively charged coat that contributes as repulsive molecules in leukocyte-endothelial interaction. The present study aims to clarify whether endothelial glycocalyx thickness may change in the retinal and choroidal microvasculature in diabetic or hypertensive rats, and to examine whether leukocyte-endothelial adhesion may be only in areas of the cell surface relatively depleted of leukocyte and endothelial glycocalyx.

Methods:: We used Wistar Kyoto rats (WKY) as a control (n = 5), streptozocin induced diabetic rats (STZ-WKY) (n = 5), and spontaneously hypertensive rats (SHR) (n = 5). We perfused the eye with a cacodylate-buffered fixatives (pH7.3) containing cationic colloidal iron. After excision of the eye, we postfixed the eye with 1% osmium tetroxide and embedded in epoxy resin. Then we cut the specimen into ultrathin sections. We observed endothelial glycocalyx in retinal and choroidal microvasculature under a transmission electron microscope and quantified its thickness by using NIH image software.

Results:: Negatively charged glycocalyx was intensely stained with cationic colloidal iron in WKY. The thickness of endothelial glycocalyx was significantly decreased in the retinal and choroidal microvessels of STZ-WKY and SHR. The glycocalyx thickness in leukocyte-endothelial adhesion areas was significantly decreased both on leukocytes and endothelial cells compared with that in free areas in the choroidal vasculature of STZ-WKY, though we could not find such adhesion sites in SHR.

Conclusions:: These findings suggest that endothelial glycocalyx disruption is induced in both diabetic and hypertensive rats and that some glycocalyx disruption is dependent on the process of leukocyte-endothelial cell adhesion in diabetic rats. In addition, according to no leukocytes adherent to endothelial cells in SHR, glycocalyx changes may be also general processes modulating leukocyte-endothelial adhesion unrelated to specific receptors.

Keywords: cell adhesions/cell junctions • proteoglycans/glycosaminoglycans • diabetic retinopathy 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×