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T. T. Lam, B. Pfeffer, L. McMullen, J. Zhang; Simultaneous Determination of Several Cytokines/Chemokines From Immortalized Human Retinal Pigment Epithelial Cells (ARPE-19) Under Normal and Hypoxic Conditions by a Multiplex System. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5048.
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This study was designed to simultaneously determine several cytokine/chemokine levels from ARPE-19 cells, an immortalized human retinal pigment epithelial cell line, under normal and hypoxic conditions by a multiplex system.
Human retinal pigment epithelial cells (ARPE-19) were cultured under normal and hypoxic (1% oxygen) conditions for 24 hours. Cytokine/chemokine levels in the culture medium were analyzed using a multiplex system (Luminex).
Substantial levels of VEGF, IL-6, IL-8, and MCP-1 were simultaneously detected in the culture medium of ARPE-19 under normal culture conditions. Hypoxia upregulated VEGF but downregulated the other three.
While the upregulation of VEGF under hypoxia and its pathophysiological roles are well studied, the pathological significance of the downregulation of IL-6, IL-8 and MCP-1 from ARPE-19 remains to be examined. This study also suggests that a multiplex system provides a convenient means to determine the relative levels of several biomolecules simultaneously with small amounts of samples.
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