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E. L. West, R. A. Pearson, J. C. Sowden, R. E. MacLaren, R. R. Ali; The Outer Limiting Membrane as a Barrier to Photoreceptor Integration, After Transplantation to the Subretinal Space of the Adult Mouse. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5080.
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© ARVO (1962-2015); The Authors (2016-present)
To determine whether the presence of the outer limiting membrane (OLM) in adult retinas limits the integration and differentiation of transplanted immature retinal cells.
A transient chemical disruption of the OLM was induced in adult mice. Cells from dissociated P1 neural retinas were transplanted into the subretinal space at various timepoints after chemical administration. At 3 weeks post injection, the number of integrated, differentiated photoreceptor cells present in the OLM disrupted eyes, was compared to the PBS-treated contralateral eye.
The disrupted retina recovered normal morphology 2 weeks post chemical administration and the OLM reformed. A significant increase in cell integration was observed in the chemically disrupted, compared with PBS treated, eyes at the timepoint that corresponded to the peak of OLM disruption.
The presence of the OLM is a limiting factor with regard to photoreceptor transplantation in the adult mouse.
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