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N. Tojo, Y. Kashiwagi, K. Nishizuka, S. Yamamoto, N. Sugawara, T. Yamashita, T. Yamamoto, H. Yamashita; Expression Regulation of VEGF and IL-6 by Inflammatory Cytokines Hyalocytes. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5163.
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© ARVO (1962-2015); The Authors (2016-present)
Hyalocytes are involved in the pathogenesis of proliferative vitreo-retinal disease. Inflammatory cytokines exert certain effects on hyalocytes and play important roles in proliferative diseases, including diabetic retinopathy. To investigate the effects of inflammatory cytokines on hyalocytes, we observed the expression regulation of vascular endothelial growth factor (VEGF) and interleukin6 (IL-6) by inflammatory cytokines in a porcine hyalocytes.
We used the primary culture of the hyalocytes derived from porcine vitreous and the established cell line derived from hyalocytes of porcine eyes (Nishizuka K et al: ARVO meeting in 2006). Two types of hyalocytes were exposed by interleukin-1alpha(IL-1α), interleukin-1beta (IL-1ß), transforming growth factor (TGFß), platelet derived growth factor AA (PDGF-AA), platelet derived growth factor BB (PDGF-BB), tumor necrosis factor alpha(TNFα) for 6-48hours. We observed the expression levels of VEGF and IL-6 at mRNA level (RT-PCR) and at protein levels (ELISA). We also checked the effects of Interleukin 1 receptor antagonist (IL-1Ra).
The expression of VEGF and IL-6 was stimulated significantly only by IL-1α. IL-6 did not affects the expression levels of VEGF. IL-1Ra inhibited the expression level increase of VEGF and IL-6 induced by IL-1α. These responses were similar in both the primary culture hyalocytes and the hyalocyte cell line.
The expression of VEGF and IL-6 was increased by the stimulation of IL-1α in porcine hyalocytes, but not by IL-1ß. IL-1α is a pro-inflammatory cytokine and central mediator in cytokine network, and may contribute to the development of proliferative vitreo-retinal diseases through the functions of hyalocytes.
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