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K. Kojima, M. Ueta, J. Hamuro, N. Yokoi, S. Kinoshita; Expression and Function of TLR2 in Human Conjunctival Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5173.
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© ARVO (1962-2015); The Authors (2016-present)
The ocular surface, composed of corneal and conjunctival epithelial cells, is constantly exposed to microbes. It is reported by many groups that corneal epithelial cells express functional toll-like receptors (TLRs), which play an important role in innate immunity by producing cytokines and antimicrobial peptides upon recognizing microbial-associated molecular patterns (MAMPs). We previously reported that human corneal epithelial cells express toll-like receptor 2 (TLR2), but that they respond to only limited kinds of TLR2 ligands. In this study, we investigated the expression and function of TLR2 in human conjunctival epithelial cells in vitro.
Human conjunctival epithelium samples harvested at the time of surgery were immunohistochemically examined for TLR2. Expression of TLR2 in conjunctival epithelial cells was studied by RT-PCR and flow cytometry. The cultured immortalized human conjunctival epithelial cell line (HCjE-T) was stimulated with TLR2 ligands, purified lipoteichoic acid from Staphylococcus aureus (LTA-SA), and synthetic lipoprotein (FSL-1). The HCjE-T was also stimulated with IL-1α as a positive control. The amount of IL-6 and IL-8 in supernatants harvested after twenty-four hours of exposure to these ligands was measured by ELISA. IL-6 and IL8 mRNA expressions of HCjE-T after two hours of exposure to each ligand were examined by quantitative RT-PCR.
TLR2 was expressed intracellularly in conjunctival epithelial cells. HCjE-T stimulated with LTA-SA and FSL-1 showed increased production and mRNA upregulation of IL-6 and IL-8 by ELISA and quantitative RT-PCR compared to un-stimulated HCjE-T.
HCjE-T expressed functional TLR2s, and ocular surface epithelium possesses functional TLR2s.
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