May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Human Iris Pigment Epithelial (hIPE) Cells Possess a Capacity to Modulate T Cell Activation
Author Affiliations & Notes
  • T. Hattori
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • T. Kezuka
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • Y. Usui
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • M. Takeuchi
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • Y. Okunuki
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • M. Kurita
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • R. Yamashita
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • M. Haneda
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • S. Shirato
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • M. Usui
    Ophthalmology, Tokyo Medical University, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships T. Hattori, None; T. Kezuka, None; Y. Usui, None; M. Takeuchi, None; Y. Okunuki, None; M. Kurita, None; R. Yamashita, None; M. Haneda, None; S. Shirato, None; M. Usui, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5194. doi:
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    • Get Citation

      T. Hattori, T. Kezuka, Y. Usui, M. Takeuchi, Y. Okunuki, M. Kurita, R. Yamashita, M. Haneda, S. Shirato, M. Usui; Human Iris Pigment Epithelial (hIPE) Cells Possess a Capacity to Modulate T Cell Activation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5194.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To determine whether Human iris pigment epithelial (hIPE) cells have a capacity to suppress T cell activation. And if so, mechanism of suppression capacity was revealed.

Methods:: Human iris pigment epithelial (hIPE) cells were prepared from Glaucoma patients undertaken surgery, and were incubated in vitro 4-7 days in RPMI1640 medium containing 10% FCS. Expression of MHC molecules and costimulatory molecules on cultured hIPE cells stimulated with or without IFN-g were examined by FACS. In addition, periphral T cells were incubated with cultured hIPE cells prepared from the same patients and anti-CD3 antibody in transwell culture system, or in the presence of anti-B7-H1 and B7-DC antibodies, and T cell proliferation was assessed by [3H]-thymidine incorporation.

Results:: Cultured hIPE cells expressed MHC Class I, B7-H1, and B7-DC, and these expression were enhanced and MHC Class II was newly expressed by stimulation with IFN-g. Anti-CD3-driven T cell proliferation was inhibited by cultured hIPE cells, which inhibition was not observed in transwell culture system, or in the presence of anti-B7-H1 and B7-DC antibodies.

Conclusions:: Cultured hIPE cells prevent T cells proliferation via cell-to-cell contact, in which B7-H1 and B7-DC pathways are related.

Keywords: immune tolerance/privilege • iris • cell-cell communication 
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