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W. Herrmann, K. Kobuch, K. Hufendiek, P. Prahs, H. Sachs, C. P. Lohmann, H. Helbig; Epi-Lasik: A Comparative Evaluation of Epithelial Separation With 3 Different Microkeratomes. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5330.
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The aim of this study was to compare the effect of epithelial separation with 3 different Epi-LASIK microkeratomes on corneal cytoarchitecture and epithelial cell viability.
Epithelial separation was performed in 10 porcine eyes and 2 human donor eyebank eyes each with 3 different microkeratomes which differ in blade design and blade material (AMO Amadeus 2, Gebauer Epivision, Gebauer Prototype). Morphological changes and viability of epithelial cells was evaluated by light microscopy, electron microscopy, immunohistochemistry (Collagen VII, Integrin ß4) and trypan blue dye viability test.
A reproducible epithelial separation with a smooth surface was achieved in all eyes. No laceration of Bowman's layer was observed with any of the microkeratomes. In all eyes collagen VII was found on the stromal side after epithelial separation. Damage to epithelial cells was mostly limited to the cut margins The plane of separation with all microkeratomes was between lamina lucida and lamina densa.
Mechanical separation of the epithelial sheet in Epi-LASIK is safe and reproducible with all evaluated microkeratomes.
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