May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Effects of Contact Lenses on the Protein Composition in Tear Film: A Proteinchip Study
Author Affiliations & Notes
  • C. A. Kramann
    Department of Ophthalmology, Johannes Gutenberg University, Mainz, Germany
  • N. Wiegel
    Department of Ophthalmology, Johannes Gutenberg University, Mainz, Germany
  • N. Pfeiffer
    Department of Ophthalmology, Johannes Gutenberg University, Mainz, Germany
  • F. H. Grus
    Department of Ophthalmology, Johannes Gutenberg University, Mainz, Germany
  • Footnotes
    Commercial Relationships C.A. Kramann, None; N. Wiegel, None; N. Pfeiffer, None; F.H. Grus, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5408. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      C. A. Kramann, N. Wiegel, N. Pfeiffer, F. H. Grus; Effects of Contact Lenses on the Protein Composition in Tear Film: A Proteinchip Study. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5408.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: The aim of this study was to analyze the effects of hard and soft contact lenses on the protein composition of tears in contact lens wearers.

Methods:: Wearers of soft contact lenses (n=13) and hard contact lenses (n=13) were recruited. 13 non-contact lens wearers were also included as control. Tears were collected using Schirmer strips with anesthesia and frozen until use. The tears were eluted and analyzed on ProteinChips Seldi-TOF (Surface-enhanced laser-desorption and ionization in time of flight mass spectrometry; BioRad, USA) with different chromatographic surfaces (cationic and anionic exchanger, and reversed phase surface). The Seldi spectra were analyzed by multivariate statistical analysis and artificial neural networks in order to find a biomarker panel which differentiate best between the groups. Some of these protein biomarkers were identified using Maldi-TOFTOF.

Results:: The tear protein composition differed significantly from protein composition in non-contact lens wearers in both wearers of hard and soft contact lenses (p<0.01). However, the deviation of tear protein patterns were more pronounced in wearers of soft contact lenses. Nevertheless, considering typical dry-eye biomarkers found in previous studies (Grus et al., IOVS 2005), e.g. the PRP4 (proline rich protein) differed in both soft and hard contact lens wearers in comparison to the non-contact lens wearers.

Conclusions:: Contact lens wear alters the protein profiles in a complex manner. This study could demonstrate that those changes can not only be found in wearers of soft contact lenses, but also in hard contact lens wearers.

Keywords: contact lens • proteomics 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×