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Y. Kii, K. Yamakiri, Y. Sonoda, N. Doi, T. Sakamoto, T. Hashiguchi, I. Maruyama; Retinal Detachment Causes Expression of High Mobility Group Box 1 and Retinal Pigment Epithelial Cell Death. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5737.
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© ARVO (1962-2015); The Authors (2016-present)
High mobility group box 1 (HMGB1), a nuclear and cytosolic protein found as a transcription factor, has recently been identified as a cytokine mediator. We investigated a pathogenic role of HMGB1 in the development of retinal detachment (RD).
Vitreous specimens were collected from 11 eyes of 11 patients undergoing pars plana vitrectomy for RD. Vitreous specimens were collected from 11 eyes of 11 patients of macular hole (MH) or epiretinal membrane (ERM) as control. The vitreous levels of HMGB1 were measured using enzyme-linked immunodorbent assay. Immnohistochemical analysis was employed to examine localization of HMGB-1 in RD retina. Recombinant HMGB1 was added to ARPE19 cells. The secretion of proinflammatory cytokines was measured using ELISA and flow cytometry. Cell viability was tested using MTT assay and cell death was tested using PI staining.
HMGB1 concentrations were significantly higher in vitreous fluid of RD (4.75±4.51 ng/ml) than in that of MH or ERM (0.28±0.50 ng/ml). Immunohistochemistry showed that HMGB1 localized in detached retina. As recombinant HMGB1 was added to ARPE19 cells, the secretion of IL-6, IL-8, VEGF significantly increased. A marked decrease in cell viability and a marked increase in cell death were also observed.
HMGB1 plays a pathogenic role in the development of RD. HMGB1 might be a target for a new treatment of vitreous and retinal disorders.
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