May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Effect of Pathlength on Trans-Scleral Macromolecular Diffusion
Author Affiliations & Notes
  • J. Singh
    St Thomas Hospital, Rayne Institute, London, United Kingdom
  • T. L. Jackson
    St Thomas Hospital, Rayne Institute, London, United Kingdom
  • O. A. Anderson
    St Thomas Hospital, Rayne Institute, London, United Kingdom
  • A. A. Hussain
    St Thomas Hospital, Rayne Institute, London, United Kingdom
  • J. Marshall
    St Thomas Hospital, Rayne Institute, London, United Kingdom
  • Footnotes
    Commercial Relationships J. Singh, None; T.L. Jackson, None; O.A. Anderson, None; A.A. Hussain, None; J. Marshall, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5826. doi:
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      J. Singh, T. L. Jackson, O. A. Anderson, A. A. Hussain, J. Marshall; Effect of Pathlength on Trans-Scleral Macromolecular Diffusion. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5826.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To quantify the effect of pathlength (scleral thickness) on trans-scleral albumin diffusion.

Methods:: Anterior superotemporal scleral specimens were obtained from 12 donor eyes. Sclera was dissected to obtain inner sclera, outer sclera, and full-thickness sclera for each eye. Scleral specimens were mounted in a modified Ussing chamber with fluorescein isothiocyanate labelled, 66kDa, bovine albumin in the hemichamber facing the internal scleral surface. The rate of trans-scleral diffusion was determined over 24 hours, at 25°C, with a spectrophotometer. Scleral thickness was measured using gluteraldehyde fixed sections and a sliding graticule, taking the mean of 15 randomly selected sections for each specimen.

Results:: Mean scleral thickness (+/-1SD) was 547+/-167, 629+/-145, and 761+/-183 microns for inner, outer, and full-thickness sclera respectively. Trans-scleral diffusion was 15.7+/-5.3, 15.7+/-10.7, and 9.9+/-3.5 x10-3 picomoles/mm2/hr respectively. There was no significant difference in permeability comparing inner and outer sclera. There was an inverse linear relationship between pathlength and the rate of diffusion (p 0.008).

Conclusions:: Quantifying the transscleral diffusion of a 66 kDa protein and its relationship to scleral thickness may guide novel strategies of drug delivery. In particular, these data suggest that reduced scleral thickness should improve the trans-scleral delivery of high molecular weight agents.

Keywords: sclera • anatomy 
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