Purchase this article with an account.
T.-I. Kim, Y. Byoun, S. Choi, S. Kim, J. Park, H. Lee, E. Kim; Induction of ßIg-h3 Protein by Tgfß1 in Corneal Epithelial Cells and Fibroblasts. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5865.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To compare the production of ßig-h3 protein and its induction by TGFß1 in normal corneal epithelial cells and cultured corneal fibroblasts of normal and granular corneal dystrophy (GCD) type II heterozygote and homozygote.
0, 10, and 20ng/ml of TGFß1 were treated in corneal epithelial cells and 5th-6th generation corneal fibroblasts of both normal and GCD type II heterozygote and homozygote in FBS(fetal bovine serum) free culture media, then western blot and RT-PCR were followed to measure the level of ßig-h3 protein.
Increased production of ßig-h3 protein was observed in both corneal epithelial cells and fibroblasts of all three groups after 24 hours of incubation and it was directly related to the concentration of and time treated with TGFß1. Level of mRNA transcription of ßig-h3 protein was comparable in both corneal epithelial and fibroblasts and its production was increased after treatment with TGFß1.
Both corneal epithelial cells and fibroblasts of normal and GCD type II homozygotes and heterozygotes produced ßig-h3 protein, and TGFß1 was a inducing factor of ßig-h3 protein production.
This PDF is available to Subscribers Only