May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Bves, a Tight Junction Regulatory Protein, Is Increased in an Immortalized Glaucomatous TM Cell Line
Author Affiliations & Notes
  • F. R. Haselton
    Vanderbilt University, Nashville, Tennessee
    Biomedical Eng Box 1510B,
  • P. Russ
    Vanderbilt University, Nashville, Tennessee
    Biomedical Eng Box 1510B,
  • M. Chang
    Vanderbilt University, Nashville, Tennessee
    Ophtalmology & Visual Sciences,
  • Footnotes
    Commercial Relationships F.R. Haselton, None; P. Russ, None; M. Chang, None.
  • Footnotes
    Support NIH Grant EY017185
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5909. doi:
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      F. R. Haselton, P. Russ, M. Chang; Bves, a Tight Junction Regulatory Protein, Is Increased in an Immortalized Glaucomatous TM Cell Line. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5909.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: The goal of this work is to examine two trabecular meshwork (TM) cell lines for differences in junction protein content, particularly the tight junction regulator Bves, and in cell function.

Methods:: Two SV40 immortalized human TM cell lines, one from a glaucomatous donor (GTM) and one from a donor with normal ocular pressure (NTM), were obtained from Alcon. Western blotting was used to assay for the presence of Bves and other tight junction proteins. Time lapse photography was employed to study cell motility. A 2-chamber permeability assay was used to compare barrier function between the 2 cell lines.

Results:: Bves protein was found to be significantly increased in GTM cells compared to NTM cells. Additionally, expression of ZO-1 and other tight junction proteins was increased in GTM cells. It appeared that the increased tight junction components were functional, as cell motility was decreased by 65% in GTM compared to NTM, and permeability to fluorescein was decreased by 20%.

Conclusions:: Bves, a tight junction regulatory protein, is increased in an immortalized glaucomatous TM cell line. Increased tight junction protein expression, decreased cell motility, and decreased monolayer permeability were also observed in GTM cells compared to NTM cells.

Keywords: trabecular meshwork 
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