May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Mapping of the Wavy With Open Eye Lids 2 (woe2) Locus in Mice
Author Affiliations & Notes
  • L. Liang
    Medical university of Wisconsin, Milwaukee, Wisconsin
    The Department of Ophthalmology,
  • J. Toonen
    Medical university of Wisconsin, Milwaukee, Wisconsin
    The Department of Cell Biology, Neurobiology, & Anatomy,
  • L. Jackson
    Medical university of Wisconsin, Milwaukee, Wisconsin
    The Department of Ophthalmology,
  • J. Besharse
    Medical university of Wisconsin, Milwaukee, Wisconsin
    The Department of Cell Biology, Neurobiology, & Anatomy,
  • D. J. Sidjanin
    Medical university of Wisconsin, Milwaukee, Wisconsin
    The Department of Ophthalmology, The Department of Cell Biology, Neurobiology, & Anatomy,
  • Footnotes
    Commercial Relationships L. Liang, None; J. Toonen, None; L. Jackson, None; J. Besharse, None; D.J. Sidjanin, None.
  • Footnotes
    Support NEI/NIH Grant EY015173, NEI Grant EY07758, a Core Grant for Vision Research at the Medical College of Wisconsin(P30EY01931), The Morris Animal Foundation Grant D05CA-049.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5999. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      L. Liang, J. Toonen, L. Jackson, J. Besharse, D. J. Sidjanin; Mapping of the Wavy With Open Eye Lids 2 (woe2) Locus in Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5999.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: Waved with open eyes 2 (woe2) is an autosomal recessive mouse mutant that arose spontaneously on 129 X C57BL/6 background. The purpose of this study is to identify the mutation responsible for the woe2 phenotype.

Methods:: The woe2 mice were evaluated with a slit lamp biomicroscope and an indirect ophthalmoscope following mydriasis with 1% atropine. For linkage analysis woe2 mice were outcrossed to wild type C3A.BLiA-Pde6b+/J. The F1 mice were backcrossed to woe2 and 80 F2 progeny were generated. At weaning the progeny were evaluated for the coat and eye phenotype and were euthanized. Genomic DNA was isolated from collected spleens. Microsatellite markers were selected to evenly cover the mouse genome. Genotypes were determined by PCR amplification of genomic DNA and alleles were scored.

Results:: Phenotypically, the woe2 mice show wavy coat, eyelids open at birth, microphthalmia/anophthalmia, corneal opacity, corneal neovascularization and white retinal spots. The woe2 locus was mapped to 3.4 cM region on mouse chromosome 7. The linkage map established the woe2 critical region between D7Mit340 and D7Mit224. Evaluation of the mouse genome map (http://genome.ucsc.edu/cgi-bin/hgGateway) identified Protein Phosphatase 1, Regulatory (inhibitor) Subunit 13 Like (Ppp1r13l) as a candidate gene.

Conclusions:: The woe2 mouse is a mutation in a gene that plays an essential role in the normal eye development. Our mapping results established linkage of the woe2 locus to centromeric end of mouse chromosome 7. Evaluation of the genes in the critical region identified Ppp1r13l as a candidate gene. Sequencing of the Ppp1r13l gene in the woe2 mouse is currently under way

Keywords: gene mapping • gene screening 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×