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Y. Ozawa, T. Kurihara, N. Nagai, T. Koto, S. Satofuka, M. Inoue, Y. Oike, K. Tsubota, S. Ishida, H. Okano; Stat3–Activation Negatively Correlates With Rhodopsin–Expression in Adult Retina with Inflammation . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1065.
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We have previously reported that STAT3–activation negatively controls Rhodopsin–expression in perinatal retina during developmental processes (Ozawa et al. Molecular and Cellular Neuroscience 2004). Next interest is whether this regulation is also available in adult. In this study, we evaluate the level of STAT3–activation and Rhodopsin–expression during severe inflammation in adult retina.
Lipopolysaccharide (LPS) was injected into adult C57/B6 mice intrapelitoneally to establish the model of retinal inflammation (a model of endotoxin–induced uveitis) in which IL–6 is known to be upregulated. Levels of STAT3–activation and Rhodopsin–expression were analyzed in each time point after LPS injection. Dark–adapted full–field electroretinography (ERG) was recorded at 24 hours after LPS injection.
STAT3–activation was upregulated from 3 hours after intraperitoneal injection of LPS, peaking at 8 hours and decreased but still continued at 48 hours. Correlated to this, Rhodopsin–expression started to be downregulated at 16 hours and continuously decreased. Dark–adapted full–field electroretinography (ERG) clearly showed reduction in amplitude of a–wave which represents function of photoreceptor cells.
Strong STAT3–activation inhibited Rhodopsin–expression in adult retina with inflammation. Although the mechanism of negative effect of STAT3–activation on Rhodopsin–expression could be regulated by multiple stimuli at inflammation, excess STAT3–activation negatively correlated with maintainance of Rhodopsin–expression thus showed abnormality in ERG.
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