May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Endocapsular Lens Epithelial Cell Kill by Hyperthermia: A Feasibility Study
Author Affiliations & Notes
  • M.A. Orozco
    Univ.of Miami Miller School of Medicine, Miami, FL
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute,
    Biomedical Engineering, Univ. of Miami College of Engineering, Coral Gables, FL
  • I. Nose
    Univ.of Miami Miller School of Medicine, Miami, FL
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute,
  • W. Lee
    Univ.of Miami Miller School of Medicine, Miami, FL
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute,
  • A.C. Acosta
    Univ.of Miami Miller School of Medicine, Miami, FL
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute,
    Hospital Oftalmológico Dr P Lagleyze, Buenos Aires, Argentina
  • N. Salas
    Univ.of Miami Miller School of Medicine, Miami, FL
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute,
    Biomedical Engineering, Univ. of Miami College of Engineering, Coral Gables, FL
  • M. Fragoso
    Univ.of Miami Miller School of Medicine, Miami, FL
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute,
    Cell Biology and Anatomy,
  • E. Arrieta
    Univ.of Miami Miller School of Medicine, Miami, FL
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute,
    Hospital Univ. de los Andes, Merida, Venezuela
  • R. Augusteyn
    Vision CRC, Sydney, Australia
  • J.–M. Parel
    Univ.of Miami Miller School of Medicine, Miami, FL
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute,
  • Footnotes
    Commercial Relationships  M.A. Orozco, None; I. Nose, None; W. Lee, None; A.C. Acosta, None; N. Salas, None; M. Fragoso, None; E. Arrieta, None; R. Augusteyn, None; J. Parel, None.
  • Footnotes
    Support  Fl Lions Eye Bank; Australian Gov. CRC Scheme; NIH center grant P30–EY014801; Research to Prevent Blindness; Henri & Flore Lesieur Foundation
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 643. doi:
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    • Get Citation

      M.A. Orozco, I. Nose, W. Lee, A.C. Acosta, N. Salas, M. Fragoso, E. Arrieta, R. Augusteyn, J.–M. Parel; Endocapsular Lens Epithelial Cell Kill by Hyperthermia: A Feasibility Study . Invest. Ophthalmol. Vis. Sci. 2006;47(13):643.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the feasibility of lens epithelial cell (LEC) kill for prevention of anterior and posterior capsular opacification using an endocapsular heat delivery probe.

Methods: : 10 rabbit and 10 human eyes no more than 3 days post mortem were used (control eyes: 1 rabbit , 4 human; hyperthermia: 9 rabbits, 6 human). After doing a capsulorhexis (5mm for human, 3mm for rabbit) the lens matter was removed and the capsule was filled with high viscosity sodium hyaluronate. The lens was placed in a BSS filled controlled temperature chamber set at 37°C. Endocapsular hyperthermia was done with a 19ga (1mm) custom made thermal probe encapsulated in Teflon. A thermocouple within the tip allows monitoring and temperature feedback control. The heat treatment in the lens capsule was monitored with 3 external thermocouples positioned at the anterior, posterior, and equator of the lens capsule. The temperature of the capsule contents was raised to 48°C (below capsule shrinkage threshold of 51°C) and treatment was stopped. After treatment, the lens capsule was removed and cultured in DMEM containing 10% FBS, antibiotics and antimycotics at 37°C and 5% CO2. LECs viability was assessed by means of a viability assay at 0, 1, 3, and 7 days post–treatment.

Results: : In human eyes, the temperature reached 48 °C in 155±66, 195±146 and 186±64 s at the anterior, equator, and posterior capsule. In rabbit eyes the duration was 16±5, 31±20, and 19±13 s respectively. The viability assay performed at the forementioned days in culture shows a marked difference in the viability of cells dependent on the location of the probe within the capsule. The side of the capsule where the probe was located showed a higher percentage of dead cells compared to the opposite location of the probe. The percentage of dead cells (60%–90%) following treatment is significantly higher relative to the control eye (20%–40%). The percentage of live to dead cells in the treated eye will be normalized relative to the control eye.

Conclusions: : The study demonstrates the in vitro feasibility of applying hyperthermia for LEC cell kill without capsular shrinkage.

Keywords: cataract • posterior capsular opacification (PCO) • apoptosis/cell death 
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