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M.A. Orozco, I. Nose, W. Lee, A.C. Acosta, N. Salas, M. Fragoso, E. Arrieta, R. Augusteyn, J.–M. Parel; Endocapsular Lens Epithelial Cell Kill by Hyperthermia: A Feasibility Study . Invest. Ophthalmol. Vis. Sci. 2006;47(13):643.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the feasibility of lens epithelial cell (LEC) kill for prevention of anterior and posterior capsular opacification using an endocapsular heat delivery probe.
10 rabbit and 10 human eyes no more than 3 days post mortem were used (control eyes: 1 rabbit , 4 human; hyperthermia: 9 rabbits, 6 human). After doing a capsulorhexis (5mm for human, 3mm for rabbit) the lens matter was removed and the capsule was filled with high viscosity sodium hyaluronate. The lens was placed in a BSS filled controlled temperature chamber set at 37°C. Endocapsular hyperthermia was done with a 19ga (1mm) custom made thermal probe encapsulated in Teflon. A thermocouple within the tip allows monitoring and temperature feedback control. The heat treatment in the lens capsule was monitored with 3 external thermocouples positioned at the anterior, posterior, and equator of the lens capsule. The temperature of the capsule contents was raised to 48°C (below capsule shrinkage threshold of 51°C) and treatment was stopped. After treatment, the lens capsule was removed and cultured in DMEM containing 10% FBS, antibiotics and antimycotics at 37°C and 5% CO2. LECs viability was assessed by means of a viability assay at 0, 1, 3, and 7 days post–treatment.
In human eyes, the temperature reached 48 °C in 155±66, 195±146 and 186±64 s at the anterior, equator, and posterior capsule. In rabbit eyes the duration was 16±5, 31±20, and 19±13 s respectively. The viability assay performed at the forementioned days in culture shows a marked difference in the viability of cells dependent on the location of the probe within the capsule. The side of the capsule where the probe was located showed a higher percentage of dead cells compared to the opposite location of the probe. The percentage of dead cells (60%–90%) following treatment is significantly higher relative to the control eye (20%–40%). The percentage of live to dead cells in the treated eye will be normalized relative to the control eye.
The study demonstrates the in vitro feasibility of applying hyperthermia for LEC cell kill without capsular shrinkage.
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