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K.R. Mendis; Giant Cell Arteritis: An Immuno–Histochemical and a Structural Survey . Invest. Ophthalmol. Vis. Sci. 2006;47(13):784.
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© ARVO (1962-2015); The Authors (2016-present)
To quantify the dendritic cell content, the distribution of macrophage lysosomal CD 68 positive cells and the extent of internal elastic lamina (IEL) disruption in giant cell arteritis (GCA).
This was a histological retrospective study of archival paraffin embedded temporal artery sections comparing biopsy positive GCA (n=50) and age matched biopsy negative controls (n=50). Dendritic cell (DC) numbers and CD 68+ cell distribution were assessed using antibodies to S–100 and CD 68 antigens. IEL disruption was analysed by elastic Van Gieson (EVG) stain. Statistical analysis of data was by Mann–Whitney test.
A significant increase in median counts of S–100+ cells, were observed in GCA (P=0.0001) compared to controls. CD 68+ staining distribution correlated well with that of S–100 + cells but with more numerous and confluent pattern in GCA sections and no staining detected in controls. Chi–square analysis revealed a significant increase in extent of IEL disruption in GCA (P<0.0001).
S–100+ cell population is increased with a parallel and a larger influx of lysosomal CD 68+ cells in GCA. Resident S–100+ cell numbers show minimal variation compared to larger variability of S–100+ response in GCA. IEL disruption is a significant finding in GCA, which is mostly segmental and confined to the area of inflammatory infiltration.
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