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H. Bando, K.G. Shadrach, M.E. Rayborn, J.W. Crabb, J.G. Hollyfield; Clathrin and Adaptin in Drusen, Bruch's Membrane and Choroid in AMD and Non–AMD Donor Eyes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):859.
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Clathrins were identified in our recent proteomic analysis of drusen from age–related macular degeneration (AMD) donor eyes. This study was conducted to define the localization of clathrins in drusen, Bruch’s membrane and choroid of AMD tissues and to compare this distribution with that in non–AMD control tissues. Additionally, we investigated the distribution of adaptin, which is closely linked to the function of clathrin.
Human eyes were from donors between 66 and 94 years of age; 10 eyes were from donors with AMD and 10 from non–AMD donors. Bruch’s membrane and choroid from the macula from each donor eye were prepared for immunohistochemistry and Western blotting and differences in immunoreactivity were quantitated.
Drusen, Bruch’s membrane and choroid from AMD tissues showed greater immunoreactivity for clathrin and adaptin than did non–AMD tissues. Western blots also showed more intense clathrin and adaptin signals from AMD than were present in non–AMD tissues.
Clathrin and adaptin appear to be more abundant in drusen, Bruch’s membrane, choroid of AMD compared to non–AMD controls. This study suggests that accumulation of clathrin and adaptin in drusen, Bruch’s membrane and choroid may reflect a higher rate of clathrin mediated endocytosis in AMD tissues. Alternatively, the accumulation of these proteins may reflect a higher susceptibility to oxidative damage.
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