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M.C. Wills, C. Flaxel, T. Hack, T. Fenn; Characterization of Optimal Laser Parameters in a Primate Model of Experimental Choroidal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2006;47(13):902.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the relationship between laser power and choroidal neovascularization (CNV), as measure by fluorescein leakage, in Cynomolgus monkeys in order to determine optimal laser parameters.
Three cynomolgus monkeys received laser treatment in each eye to induce choroidal neovascularization. Laser treatment was conducted with a green laser (532 nm) according to the following parameters: 75 µm spot size, 100 ms duration. For each of 6 eyes, the laser power was incrementally increased from 250 mW to 600 mW. Evaluation of CNV development was achieved with fluorescein angiography. Lesions that developed a luminescent circle were counted as CNV.
The rate of CNV development ranged from 22% to 77% when assessed two weeks after laser treatment. The initial response was lowest at 250 and 350 mW. The most consistent response occurred at 500 and 600 mW. Treatment at 600 mW resulted in one area of hemorrhage; however, this hemorrhage was not associated with increased fluorescein leakage. CNV in all six eyes showed evidence of regression within 4 weeks after laser treatment, consistent with previously published data for this model.
Previous work in this lab with this model utilized a laser power range of 300–700 mW. Based on the results of this study, power settings at or below the lower end of this range may not be sufficient to induce choroidal neovascularization.
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