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S. Shippy, L. Gao, M.–J. Lu, C.A. McCannel, R.M. Hatfield, R.F. Dundervill, III, J.S. Pulido; Nitrate and Amino Acid Levels in the Vitreous of Proliferative Diabetic Retinopathy . Invest. Ophthalmol. Vis. Sci. 2006;47(13):965.
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© ARVO (1962-2015); The Authors (2016-present)
To compare the levels of nitrates and amino acids in the vitreous of patients undergoing vitrectomy for proliferative diabetic retinopathy (PDR) compared to those undergoing vitrectomy for macular hole (MH) or epiretinal membrane (ERM)
Nanoliter capillary electrophoresis (NCE) as previously described by us was used with a detection level of 100nM for glutamate and other amino acids and 1.3 microM for nitrates. 100 microL of pure vitreous taken at the time of vitrectomy was assayed with NCE. Results of those with PDR were compared by t–tests to those who had vitrectomy for MH or ERM.
Mean Nitrate levels in the vitreous of 18 eyes with MH/ERM was 16.1(+/–4.9)microM while it was 50.1(+/–21.5)microM in the vitreous of 12 eyes with PDR (p<0.001). A statistically significant difference (p<0.05) was seen in glutamate levels, 3.5(+/–2.7)microM MH/ERM (n=18) versus 8.2(+/–5.4)microM PDR (n=10). However, differences in arginine levels were not at 3.8(+/–0.5)microM for MH/ERM (n=6) and 4.4(+/–0.6)microM for PDR (n=4).
Nitrates and glutamate are significantly elevated in the vitreous of patients with PDR. Arginine levels are not significantly elevated in our sample. Nitrates are derived from nitrous oxide that is associated with capillary dilatation and this may be a pathophysiological reason why the vessels are dilated in patients with PDR. Glutamate may also be an agent in the pathophysiology of PDR.
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