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S. Mazzarella, F. Mazzolani, N. Currò, R. Ratiglia, N. Orzalesi; Comparison of Endothelial Cell Counts and Intra–Operator Variability Using Two Different Confocal Microscopes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1330.
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© ARVO (1962-2015); The Authors (2016-present)
To compare CONFOSCAN 2 (Nidek Technologies,Inc. Greensboro NC.) and HRT II with the Rostock Cornea Module ( Heidelberg Engineering GmbH, Dossenheim, Germany) in evaluating corneal endothelial cell density and manual count intra–operator variability.
20 normal eyes of 10 different patients (mean age: 23 years, range 20–26, 7 female and 3 male), were analyzed. An evaluation of the central cornea endothelial cell density was performed on the same day and by the same operator in a randomized sequence using CONFOSCAN 2 and HRT II with the Rostock Cornea Module in a manual mode. Manual count intra–operator variability was assessed on images of the same patient obtained by the same operator every hour from 8.00 a.m. to 6 p.m. (ten times). To assess the endothelial cell density and the intra–operator variability, comparable ROIs (Region of Interest) and number of counted cells were analyzed.
Manual cell count intra–operator variability was very low (mean of ten measurements 3160,5 ± 40.3 sd with HRT II with the Rostock Cornea Module and average 3260,5 ± 40.5 sd with CONFOSCAN 2 ). Mean endothelial cell density obtained with CONFUSCAN 2 was 3007,75± 382.3 cells/mm2 and by HRT II with the Rostock Cornea Module was 2921,05±316.2 cells/mm2. The difference between the two measures was not statistically significant (p<0.3 t test).
The results show no significant differences in the cell count of central cornea endothelial cells performed with CONFOSCAN 2 and HRT II with the Rostock Cornea Module. and a good reproducibility of manual cell count with both procedures.
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