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J.P. Bergmanson, J. Walsh, L. Vrazel, M. Doughty, P. Ladage, J. Harmey; A New in vitro Approach to Measure Corneal Light Transmission . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1370.
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© ARVO (1962-2015); The Authors (2016-present)
To better define the light transmission through a living cornea, a new fiber optic spectrometer method eliminating optical aberrations was developed.
Fresh corneas removed from three rabbits were placed on a 0.2mm diameter detecting fiber optic illuminated by a standard deuterium source. Light was directed to an Ocean Optics spectrometer for transmission analysis. The small beam size and the compact instrument set–up permitted experimental alteration of corneal samples efficiently and without significant loss of light through scattering.
The cornea exhibited a distinct cut off at 305nm in the UVR range but this became less pronounced as post–mortem time increased. Removing the corneal epithelium resulted in more UVR and shorter wavelengths to be transmitted. Considerable corneal UVR absorption continued despite the absence of the epithelium.
The here described apparatus efficiently gauges corneal transmission immediately post mortem and reduces many confounding variables associated with standard spectrophotometer set–ups. Our pilot data is in general agreement with reported corneal transmission curves but allowed more precise inter– and intra–corneal comparisons. Furthermore, our results demonstrated that an important portion of the total corneal UVR absorption also takes place in the corneal stroma.
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