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N. Szentmary, B. Szende, I. Süveges; P27, P63, Cd95, Bax, Bcl–2, Cathepsin and Survivin Gene Expression in Corneas of Patients With Fuchs’ Dystrophy . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1594.
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© ARVO (1962-2015); The Authors (2016-present)
To elucidate the pathways of genetically programmed cell death (apoptosis) of corneas with Fuchs' dystrophy.
Sixteen corneal buttons (14 patients, 42.8% males, age 72.3 ± 11.3 years) with Fuchs' dystrophy and 4 buttons (4 patients, 50% males, age 67.2 ± 15.9 years) from enucleated eyes with chorioideal melanoma (controls) were analysed histologically. Immunohistochemical analysis was performed to investigate the expression of p63, p27, survivin, CD95, cathepsin, bax and bcl–2.
P63 (62.5 / 100%) and P27 (100% both) were positive, bcl–2 (0% both) and bax (6.6 / 0%) were negative in both Fuchs' dystrophy and control group corneas. Survivin (46.1 / 0%) and CD95 (57.1 / 33.3%) positivity was detected more in Fuchs’dystrophy than in normal corneas. Cathepsin was expressed more frequently in Fuchs’ dystrophy corneas (90.9%) compared to controls (75%), however with weaker immunohistochemical reaction.
The genetically programmed cell death is CD95 dependent and not related to the P53 and cathepsin way in Fuchs' dystrophy corneas. Detailed regulatory mechanisms remain to be clarified.
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