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J. Lee, Y.S. In, J.–H. Ko, M.–K. Park, W.R. Wee, J.H. Lee, M.K. Kim; The Effect of MB500 on Cultured Human Corneal Epithelium: In vitro Study (II) . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1595.
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© ARVO (1962-2015); The Authors (2016-present)
We reported MB500(MDbioalpha, Daejeon, Korea) promoted the increase of hyaluronan in corneal epithelial cells, suggesting positive effect on wound healing(presented in ARVO of 2005; 2135/ B904). We investigated the effect of MB 500 on the expression of various genes associated with hyaluronan biosynthesis or mucin secretion in cultured human corneal epithelial cells.
Various concentrations(1–50uM) of MB500 were applied to the cultured human corneal epithelial cells for 48 hours and then supernatants were collected. The expression of mRNAs of hyaluronan synthase(HAS)–1, 2, 3, P2Y2, MMP–9, and UMPK was measured using RT–PCR. Semiquantitative analysis was done in a ratio of density of above genes to density of GAPDH with image analyzer (Vilberlourmat) and densitometry (Tina 2.0, Raytest, Straubenhardt, Germany).
The mean density of HAS3 gene in cells treated with 10uM MB500 was increased by 38% compared to that in cells with no treatment. The mean density of P2Y2 gene in cells treated with 5uM MB500 was enhanced by 29% compared to that in cells with no treatment. The expression of mRNA of HAS–1, 2 MMP–9, UMPK genes in cultured human corneal epithelial cells was not significantly increased after incubation with MB500.
The application of MB500 increases the expression of HAS3 and P2Y2 genes in human corneal epithelium, suggesting enhancing effect on hyaluronan synthesis and mucin secretion in dry eye.
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