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K.W. Gjorloff, S. Andreasson, A. Wackenfors, M. Malmsjö, F. Ghosh; Electrophysiological And Morphological Findings In The Rabbit Eye After Fokal Laser Photocoagulation . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1731.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate retinal function and morphological response after focal laser treatment.
12 rabbits were treated with 70 effects of laser photocoagulation in an area below the optical nerve head. The operated eyes were investigated with full–field ERG and mfERG preoperatively, and at 1, 3 and 5 weeks after surgery. The eyes were also examined immunohistochemically, using antibodies against protein kinase C (PKC) and glial fibrillay acidic protein (GFAP). PKC was additionally examined using real–time PCR.
The b–wave amplitudes for the full–field ERG exhibited normal values at 1 week after surgery. After 3 weeks the amplitudes for scotopic examinations had increased to supernormal levels for blue, red and white light, but returned to normal values again at 5 weeks. The implicit times remained unchanged at all time intervals. In the mfERG the b–wave amplitudes for the hexagons under the optic nerve head, including the lasertreated area, did not change postoperatively. The implicit time however, increased at 3 and 5 weeks. The PKC labeling was slightly reduced at 1 week, returned to almost normal values at 3 weeks but had totally disappeared by 5 weeks. The real–time PRC showed a decreased level of PKC alfa, unchanged levels of PKC beta I and a slightly increased level for PKC beta II. The Müller cells displayed intense labeling for GFAP at 1 week and slightly increased at 3 weeks. At 5 weeks no GFAP labeling was detected.
Focal retinal laser treatment does not inhibit retinal function even though morphological signs of widespread trauma is evident. The level of PKC alpha in rod bipolar cells are strongly influenced by the surgery, which may help to explain beneficial effects of the treatment in patients with diabetic retinopathy.
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