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R.A. Renard, I.B. Lobov, Y. Liu, J. Cao, K.D. Anderson, N.J. Papadopoulos, J.S. Rudge, G.D. Yancopoulos, S.J. Wiegand; Intravitreal Administration of VEGF Trap Inhibits Pathological Retinal Neovascularization in a Mouse Model of Oxygen Induced Retinopathy . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1750.
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In the Oxygen–Induced Retinopathy (OIR) model, hyperoxia induces vaso–obliteration in the central retina of neonatal mice followed by ischemia–induced pathological retinal neovascularization when animals are returned to room air. We have previously reported that systemic administration of VEGF Trap, a potent VEGF inhibitor which binds all VEGF–A isoforms and placental growth factor, blocks pathological neovascularization in this model (Invest. Ophthalmol. Vis. Sci. 2002 43: E–Abstract 3714). The present studies assess the effects of intravitreal administration of VEGF Trap on pathological retinal neovascularization in OIR.
OIR was produced following the method developed by Smith et al (Invest. Ophthalmol. Vis. Sci. 1994, 35:101–111). To assess the effect of intravitreal delivery, VEGF Trap or a control protein, hFc, was injected intravitreally (ITV) on P14, 2 days following return to room air. Eyes were enucleated at P17, and one retina was flat–mounted and stained with FITC –labeled Griffonia Simplicifolia Lectin I to visualize the retinal vasculature. The contralateral eye was embedded, sectioned and stained with hematoxylin and eosin.
Within five days following return to room air at P17, the retinas of all control mice exhibited marked pathological angiogenesis, characterized by the presence of vascular tufts penetrating the inner limiting membrane and chaotic sprouting of vessels on the surface of the retina. Intravitreal administration of VEGF Trap almost completely blocked the development of these vascular abnormalities.
Intravitreal administration of VEGF Trap efficiently suppresses pathologic retinal angiogenesis.
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