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A.A. Esposito, B. Suedekum, J. Liu, J. Lass, F. Lin, M.E. Medof; DAF and CD59 Are Essential to Suppress T Cell Responses in Corneal Transplantation . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1782.
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© ARVO (1962-2015); The Authors (2016-present)
In recent studies, we showed that DAF and CD59, originally characterized as cell surface regulators which protect self cells from complement attack, play a central role in the induction of T cell response. In other work, we showed that the two molecules are essential for anterior chamber–associated immune deviation (ACAID). To study their importance in suppressing T cell responses against corneal transplants, we measured T cell anti–male Dby and Uty responses following transplantation of male corneas from Daf1–/–CD59a–/– mice or WTs to female WT recipients, and male corneas from Daf1–/–CD59a–/– mice or WTs to female Daf1–/–CD59a–/– recipients.
2 mm corneal grafts from each of the mouse groups were transplanted using 8 interrupted sutures into 1.5 mm beds. All animals were mixed 129/C57BL/6 backcrossed 5 generations on the C57BL/6 background. Following transplantation, rejection was graded clinically, histology analyzed, and recipient CD4 and CD8 responses to male antigen measured by IFN–γ ELISPOTS to Dby and Uty respectively.
Marked responses to both CD4–restricted Dby and CD8–restricted Uty were seen in Daf1–/– transplants and Daf1–/–CD59a–/– corneas. Similarly increased results were found when WT male corneas were used and when recipients were deficient in DAF or both regulators.
DAF and CD59 on both transplants and recipients are necessary to suppress both CD4 and CD8 responses to corneal transplantation. The results suggest that administration of DAF and/or CD59 could have clinical value in high risk transplants.
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