Purchase this article with an account.
A. Ando, N. Kiriyama, S. Kaneko, T. Nishimura, M. Matsumura; Aquaporin Gene Expression in Trabecular Meshwork Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1841.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
We examined the gene expression of aquaporin (AQP), a water channel protein, and of the ion channel proteins Kir 7.1, Kir 2.1, and Na+/K+ ATPase, in human and porcine trabecular meshwork (TM) cells.
TM cells were obtained from a porcine eye, while human TM cells were obtained from a patient with primary open angle glaucoma during a trabeculectomy. The cells were cultured in F12/DME medium with 5% fetal bovine serum added and used at the second to third passage. Total RNA was extracted and reverse transcribed into cDNA. A reverse–transcribed polymerase chain reaction (RT–PCR) method was performed to detect the gene expression of AQP–1 through AQP–9, Kir 7.1, Kir 2.1, and Na+/K+ ATPase using specific primers for the cDNA sequences.
Among the molecules examined, the gene expression of AQP–1, AQP–2, AQP–3, AQP–4, AQP–5, Kir 7.1, Kir 2.1, and Na+/K+ ATPase was detectable, while the others showed only faint evidence following amplification by RT–PCR.
We considered that these molecules may play important roles in the regulatory system for intraocular pressure by controlling aqueous outflow and ion transport.
This PDF is available to Subscribers Only