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J.N. Malta, I. Romero, M.A. S. Murca, L.M. G. Mimica, P.E. C. Dantas, M.C. Nishiwaki–Dantas, R.Y. Hida; Antimicrobial Study of the Vital Staining Formulations Used in Ophthalmology . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1921.
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analyze possible antimicrobial effect of the vital staining formulations used in the ophthalmology
antimicrobial analysis of 1% methylene blue, 1% trypan blue, 1% sodium fluorescein, 1% lissamine green and 1% rose bengal were performed against Staphylococcus aureus (ATCC25924), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853) and Klebsiella pneumoniae (ATCC1388) using Muller–Hinton culture broth and Streptococcus pneumoniae (ATCC49619) using blood agar broth. Inhibition halos were measured and its bactericidal activity analysed.
trypan blue and lissamine green did not show inhibition halo in all bacteria studied. Rose Bengal showed inhibition halo in 95% of the samples for Staphylococcus aureus. Methylene blue showed inhibition halo in 40% of the samples for Staphylococcus aureus and 95% for Streptococcus pneumoniae. Sodium fluorescein showed inhibition halo in 15% of the samples for Staphylococcus aureus and 25% of the samples for Klebsiella pneumoniae. Inhibition halo was not observed in Pseudomonas aeruginosa e Escherichia coli.
Trypan blue and lissamine green did not show antimicrobial effect for any microorganisms studied. Rose Bengal, methylene blue and sodium fluorescein showed variable antimicrobial effect.
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