May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
The Antibacterial Effect in vitro of the Ethyl–Cyanoacrylate in Different Application Volumes
Author Affiliations & Notes
  • I.L. Romero
    Ophthalmology, Santa Casa de Sao Paulo, Sao Paulo, Brazil
  • T.P. Paiato
    Ophthalmology, Santa Casa de Sao Paulo, Sao Paulo, Brazil
  • L.M. G. Mimica
    Ophthalmology, Santa Casa de Sao Paulo, Sao Paulo, Brazil
  • C.B. Silva
    Ophthalmology, Santa Casa de Sao Paulo, Sao Paulo, Brazil
  • J.B. N. S. Malta
    Ophthalmology, Santa Casa de Sao Paulo, Sao Paulo, Brazil
  • P.E. C. Dantas
    Ophthalmology, Santa Casa de Sao Paulo, Sao Paulo, Brazil
  • M.C. Nishiwaki–Dantas
    Ophthalmology, Santa Casa de Sao Paulo, Sao Paulo, Brazil
  • R.Y. Hida
    Ophthalmology, Santa Casa de Sao Paulo, Sao Paulo, Brazil
  • Footnotes
    Commercial Relationships  I.L. Romero, None; T.P. Paiato, None; L.M.G. Mimica, None; C.B. Silva, None; J.B.N.S. Malta, None; P.E.C. Dantas, None; M.C. Nishiwaki–Dantas, None; R.Y. Hida, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1924. doi:
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      I.L. Romero, T.P. Paiato, L.M. G. Mimica, C.B. Silva, J.B. N. S. Malta, P.E. C. Dantas, M.C. Nishiwaki–Dantas, R.Y. Hida; The Antibacterial Effect in vitro of the Ethyl–Cyanoacrylate in Different Application Volumes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1924.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : to analyze the antibacterial effect in vitro of the ethyl–cyanoacrylate in different application volumes.

Methods: : Staphylococcus aureus (ATCC25924), Streptococcus pneumoniae (ATCC49619), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853) and Klebsiela pneumoniae (ATCC1388) were analyzed. The diameter of the drop of 4, 6, 8, 10, 12, 14, 25 and 35µl of ethyl–cyanoacrylate in the broth were measured and its area calculated. The inhibition halo caused by the antimicrobial effect of the different drop of ethyl–cyanoacrylate was measured and its area calculated. The relation between the area of the drop of the ethyl–cyanoacrylate and the area of inhibition halo were calculated allowing the comparison of the antibacterial effect in different volumes.

Results: : Inhibition halo were observed in Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli and Klebsiela pneumoniae, showing different bactericidal properties in different applied volumes. However, there was no effect on Pseudomonas aeruginosa.

Conclusions: : the in vitro bactericidal effect of the ethyl–cyanoacrylate against Staphylococcus aureus and Escherichia coli varies with its volume. There was no antibacterial effect against Pseudomonas aeruginosa. The ethyl–cyanoacrylate does not allow wide spectrum antibacterial effect in studied volumes.

Keywords: bacterial disease • microbial pathogenesis: experimental studies • Staphylococcus 
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