May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Elevation of S100 Calcium Binding Protein A8 in Tear Fluid of Pterygium Patients
Author Affiliations & Notes
  • L. Zhou
    Singapore Eye Research Institute, Singapore, Singapore
  • R.W. Beuerman
    Singapore Eye Research Institute, Singapore, Singapore
    Dept. of Ophthalmology, National University of Singapore, Singapore
  • L.P. K. Ang
    Dept. of Ophthalmology, National University of Singapore, Singapore
    Singapore National Eye Centre, Singapore, Singapore
  • C.M. Chan
    Singapore National Eye Centre, Singapore, Singapore
  • S.P. Liu
    Singapore Eye Research Institute, Singapore, Singapore
  • Y.H. Foo
    Singapore Eye Research Institute, Singapore, Singapore
  • D.T. H. Tan
    Singapore Eye Research Institute, Singapore, Singapore
    Dept. of Ophthalmology, National University of Singapore, Singapore
  • Footnotes
    Commercial Relationships  L. Zhou, None; R.W. Beuerman, None; L.P.K. Ang, None; C.M. Chan, None; S.P. Liu, None; Y.H. Foo, None; D.T.H. Tan, None.
  • Footnotes
    Support  NMRC/0808/2003, NMRC/CPG/002/2003 and NMRC IBG, Singapore
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1969. doi:
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      L. Zhou, R.W. Beuerman, L.P. K. Ang, C.M. Chan, S.P. Liu, Y.H. Foo, D.T. H. Tan; Elevation of S100 Calcium Binding Protein A8 in Tear Fluid of Pterygium Patients . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1969.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The pathogenesis of pterygia is still not well understood. Recent studies suggest that it may be associated with inflammation and progressive proliferation triggered by UV radiation. In this study we have determined that the inflammatory nature of pterygium is reflected in the protein components of the tears.

Methods: : Consent was obtained from twelve patients (average age: 57, 8 male, 4 female) with only unilateral pterygium for this study. Tears were collected using fire–polished 10 µl calibrated glass pipettes prior to pterygium surgery from diseased eyes and contralateral normal control eyes. Tear protein profiles obtained from diseased and control eyes (from seven patient samples) were compared using SELDI ProteinChip technology. Tears from another five pterygium patients were used for subsequent protein identification experiments using nanoLC coupled with nano–electrospray tandem mass spectrometry (nano–ESI–MS/MS).

Results: : SELDI mass spectra showed that a protein with the molecular weight of 10.8 kDa was found to be elevated in tears from eyes with pterygium. Elevation of this protein in tear fluid from eyes with pterygium was observed in all seven patients. This protein was purified by reverse–phase HPLC and its tryptic digest was analyzed by nanoLC–ESI–MS/MS. Two peptide fragments (LLETECPQYIR and KGADVWFK) were observed and found to be originated from S100 calcium binding protein A8. A scoring system was established for semi–quantitative analysis. The scores of 3/7 patients were +++ (> 5–fold, diseased vs control), 1/7 patient was ++ (2∼5 fold) and 3/7 patients were + (1∼2 fold).

Conclusions: : S100 A8 is known as a marker for inflammation. Elevation of S100 A8 in tears of pterygium patients may suggest a role in the pathogenesis of pterygium. It may also serve as an indicator for predicting recurrent pterygium.

Keywords: Pterygium • proteomics • cornea: tears/tear film/dry eye 
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