May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Hypothermia of 8 Degrees C Protects Cultured Retinal Pigment Epithelial Cells and Retinal Ganglion Cells Against Trypan Blue Toxicity
Author Affiliations & Notes
  • H. Kunikata
    Tohoku University Graduate School of Medicine, Sendai, Japan
    Department of Ophthalmology and Visual Science,
  • T. Abe
    Tohoku University Graduate School of Medicine, Sendai, Japan
    Division of Clinical Cell Therapy,
  • H. Murata
    Tohoku University Graduate School of Medicine, Sendai, Japan
    Department of Ophthalmology and Visual Science,
  • Y. Sagara
    Tohoku University Graduate School of Medicine, Sendai, Japan
    Department of Ophthalmology and Visual Science,
  • R. Wakusawa
    Tohoku University Graduate School of Medicine, Sendai, Japan
    Department of Ophthalmology and Visual Science,
  • H. Sato
    Tohoku University Graduate School of Medicine, Sendai, Japan
    Department of Ophthalmology and Visual Science,
  • M. Yoshida
    Department of Ophthalmology, Tohoku Kosai Hospital, Sendai, Japan
  • N. Fuse
    Tohoku University Graduate School of Medicine, Sendai, Japan
    Department of Ophthalmology and Visual Science,
  • M. Tamai
    Tohoku University Biomedical Engineering Research Organization, Sendai, Japan
  • K. Nishida
    Tohoku University Graduate School of Medicine, Sendai, Japan
    Department of Ophthalmology and Visual Science,
  • Footnotes
    Commercial Relationships  H. Kunikata, None; T. Abe, None; H. Murata, None; Y. Sagara, None; R. Wakusawa, None; H. Sato, None; M. Yoshida, None; N. Fuse, None; M. Tamai, None; K. Nishida, None.
  • Footnotes
    Support  Grant–in–Aid for the Encouragement of Young Scientists (17791216) from Japan Society for the Promotion of Science
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 2051. doi:
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      H. Kunikata, T. Abe, H. Murata, Y. Sagara, R. Wakusawa, H. Sato, M. Yoshida, N. Fuse, M. Tamai, K. Nishida; Hypothermia of 8 Degrees C Protects Cultured Retinal Pigment Epithelial Cells and Retinal Ganglion Cells Against Trypan Blue Toxicity . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2051.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether trypan blue (TB) is toxic to cultured human retinal pigment epithelial (ARPE–19) cells and rat retinal ganglion cells (RGC–5), and whether hypothermia of 8°C can protect these cells against the TB toxicity.

Methods: : ARPE–19 cells and RGC–5 were cultured and exposed to balanced salt solution (BSS) as control, and to 0.05% and 0.5% TB in BSS at 37°C and 8°C for 1 minute. The percentage of ARPE–19 cells and RGC–5 that survived in the experimental groups relative to the control group was determined by resazurin 1 day after the exposure.

Results: : TB induced a statistically significant decrease in the percentage of RPE cell surviving at 0.5% TB at 37°C (P <0.01). The percentage of surviving ARPE–19 cells in 0.05% TB at both 37°C and 8°C, and in 0.5% TB at 8°C was not significantly different from that of controls. On the other hand, TB induced a statistically significant decrease in the percentage of RGC–5 surviving at all conditions except for 0.05% TB at 8°C (0.05% 37°C; P <0.05, 0.5% 37°C and 8°C; P <0.01).

Conclusions: : These results indicate that TB is toxic to ARPE–19 cells and RGC–5 in culture, but reducing the temperature to 8°C has a protective effect against the TB toxicity.

Keywords: drug toxicity/drug effects • ganglion cells • retinal pigment epithelium 
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