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N.G. Rawlings, S. Brownstein, J. Robinson, R. Buhrmann, S. Robertson, D.R. Jordan; Immunohistochemical Localization Of Tumor Vasculature In Uveal Melanoma . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2221.
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It is widely accepted that angiogenesis plays a vital role in the pathogenesis of uveal melanoma. Selective markers of tumor microvasculature have been researched because of their possible utility in vascular targeting as well as their prognostic implications. Previous studies have examined vascular markers in various solid tumors but little has been reported in regards to uveal melanoma. We report the immunohistochemical expression of several vascular moieties in archival and prospective cases of uveal melanoma.
23 cases of retrospective and 2 cases of prospective uveal melanomas were retrieved from the ophthalmic pathology registry at the University of Ottawa Eye Institute from January 2000 to November 2005. The specimens underwent routine histochemical analysis as well as staining for CD34, Ulex europaeus agglutinin type 1, and factor VIII which localize to tumor and non–tumor endothelium. These were compared with staining for endoglin (CD105), vascular endothelial growth factor (VEGF, CD301), VEGF (CD302), prostate specific membrane antigen (PSMA), E–selectin (CD62E), and CD44 to determine if they displayed selectivity for tumor microvasculature. Three individual pathologists analyzed the specimens.
Immunohistochemical staining for CD34, Ulex, and factor VIII confirmed that all of the specimens were vascularized. There was minimal staining of CD301 and CD302 in tumor vasculature (31% and 0% respectively) but they were expressed in non–tumor vessels in 100% of the specimens. Staining for endoglin (CD105) was seen in normal vessels in 15% of the specimens and in tumor vessels in 62%. Testing for CD62E, CD44, and PSMA expression is ongoing and the results will be included in our poster.
This study demonstrates for the first time the preferential expression of endoglin in blood vessels in uveal melanoma. However, stains for VEGF (CD301 and CD302) showed no specificity for the vasculature of uveal melanoma.
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