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I. Masuda, Y. Takada, T. Igarashi, F. Kang, M. Fukushi, T. Hasegawa, Y. Yamada, K.G. Csaky; The Pathology of Lens of Perlecan Rescued Mice . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2530.
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Perlecan is a major heparan sulfatrte proteoglycan (HSPG) of basement membranes (BMs) and connective tissue. Heparan sulfate(HS) side chains in the lens capsule has been reported to have a structural function and be essential in the insulation of the lens. The aim of this study is to examine the role for core protein of perlecan in the development of cataract.
The paraffin sections were stained by hematoxylin–eosin and PAS method. The paraffin sections of neonatal mouse eyes used for In Situ TUNEL assay. Frozen sections were stained by perlecan primary antibody. The acid DNase in lens was measured. The expression of adhesion molecules(cadherin,integrin) was checked in lens.
Immunostaining for perlecan confirmed the presence of perlecan in lens in wild mice and perlecan rescued mice. PAS staining cleared the degeneration of lens capsule and the detachment of lens epithelial cells. In situ TUNEL assay and acid DNase assay are on going.
The core protein of perlecan may play a role in the development of cataract . We will investigate relationship catarct and perlecan still more.
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