Purchase this article with an account.
L. Kim, J. Oh, E. Lee, C. Xu; Inhibitory Effects of Paclitaxel and Cisplatin on Transdifferentiation of Lens Epithelial Cells Into Fibroblast in vitro Capsular Bag Culture Model . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2542.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate the inhibitory effects of Paclitaxel and Cisplatin on the transdifferentiation of the lens epithelial cells (LECs) into fibroblast using in vitro capsular bag model.
In vitro capsular bag model was prepared with porcine lens capsule and human LECs was cultured on posterior lens capsule. LECs were exposed to different concentration of paclitaxel (1, 10, and 100 nM; Group I) and cisplatin (1, 10, and 100 µM; Group II) for 3 minutes. LECs of control group as washed with DMEM in same manner. At day 7 of culture, morphological change of LECs and the number of fibroblast was compared by phase–contrast microscopy. The Inhibitory effect by paclitaxel and cisplatin on transdifferentiation of LECs into fibroblast was examined using PCNA and BrdU immunohistochemistry. Amount of type IV collagen was compared by Western blot analysis.
In group I and II, the LECs were flattened with spindle appearance. When the concentration reached 100 nM and 100 µM, in group I and II respectively, number of fibroblasts decreased significantly when compared to the control group. The number of positive cells was decreased in PCNA and BrdU immunohistochemistry in group treated with 100 nM of palitaxel and µM of cisplantin. In Western blot analysis, expression of type IV collagen was decreased in group treated with 100 nM of palitaxel and 100µM of cisplantin,
Paclitaxel and cisplatin effectively decrease the transdifferentiation of the LECs into fibroblast in vitro capsular bag model. This study suggested the possible clinical use of paclitaxel and cisplatin for the prevention of after–cataract.
This PDF is available to Subscribers Only