May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
All–Trans–Retinol Induces Rapid Recruitment of Tip47 to Lipid Droplets in the Retinal Pigment Epithelium
Author Affiliations & Notes
  • E. Tsuiki
    Department of Ophthalmology and Visual Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
  • A. Fujita
    Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Y. Ohsaki
    Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • J. Cheng
    Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • T. Irie
    Department of Anatomy, Akita University School of Medicine, Akita, Japan
  • H. Senoo
    Department of Anatomy, Akita University School of Medicine, Akita, Japan
  • K. Mishima
    Department of Ophthalmology and Visual Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
  • T. Kitaoka
    Department of Ophthalmology and Visual Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
  • T. Fujimoto
    Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Footnotes
    Commercial Relationships  E. Tsuiki, None; A. Fujita, None; Y. Ohsaki, None; J. Cheng, None; T. Irie, None; H. Senoo, None; K. Mishima, None; T. Kitaoka, None; T. Fujimoto, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 2856. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      E. Tsuiki, A. Fujita, Y. Ohsaki, J. Cheng, T. Irie, H. Senoo, K. Mishima, T. Kitaoka, T. Fujimoto; All–Trans–Retinol Induces Rapid Recruitment of Tip47 to Lipid Droplets in the Retinal Pigment Epithelium . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2856.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To determine the existence and the distribution of lipid droplets (LD) and LD proteins in the retinal pigment epithelium (RPE). Furthermore to investigate the effect of light stimulation on LD and LD proteins in the RPE, and to reveal the underlying molecular mechanism.

Methods: : We used ARPE–19 cells and the RPE of ddy mice. LDs and LD proteins, i.e., adipocyte differentiation–related protein (ADRP), TIP47, and Rab18, were examined by immunohistochemistry. Mouse eyes exposed to flashlight and ARPE–19 cells treated with all–trans–retinol were labeled for immunofluorescence microscopy and the labeling intensity was quantified by image analysis.

Results: : ADRP and TIP47 were expressed in the RPE. Flashlight on dark–adapted mouse eyes and all–trans–retinol on ARPE–19 cells caused rapid translocation of TIP47 from the cytosol to LDs, whereas ADRP distributed constitutively in LDs. Western blotting showed that TIP47 expression in RPE did not change by supplementation of all–trans–retinol.

Conclusions: : It was revealed that TIP47 in RPE accumulated to LDs in response to all–trans–retinol generated by photobleaching. We speculated that this dynamic translocation of TIP47 is functionally related to the retinoid cycle in the RPE.

Keywords: immunohistochemistry • protein structure/function • retinal pigment epithelium 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×