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R.J. Zawadzki, S.M. Jones, M. Zhao, S.S. Choi, S. Laut, B.A. Bower, S.S. Olivier, J.A. Izatt, J.S. Werner; 3D Retinal Imaging with Video–Rate Adaptive Optics Optical Coherence Tomography . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2925.
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© ARVO (1962-2015); The Authors (2016-present)
Adaptive Optics – Optical Coherence Tomography (AO–OCT) as a combination of two imaging modalities allowing high lateral (AO) and high axial (OCT) resolution, offers to date the best 3D resolution for in vivo retinal imaging. In this paper we plan to present the abilities of our enhanced AO–OCT system (using two wavefront correctors) to visualize different 3D microscopic cellular structures in the living retina.
Fourier–domain optical coherence tomography was achieved using a superluminescent diode centered at 840 nm and 50 nm optical bandwidth (6 µm axial resolution) operating at 18,000 A–scans/s (36 Frames/s, 500 A–scans/Frame) allowing a video–rate real time visualization of OCT B–scans. This system was combined with a novel closed–loop AO system to quantify and correct wavefront aberrations using a Hartmann–Shack sensor at 25 Hz and two deformable mirrors. A high–stroke 35–actuator bimorph deformable mirror for low–order aberrations correction and 144–actuator Micro Electro Mechanical Mirror for high–order aberrations correction resulted in near diffraction–limited lateral resolution (3–4 µm) and improved dynamic range of our AO system.
High 3D resolution of AO–OCT allows improved in vivo imaging of small structures lying within retinal layers and greater differentiation between retinal layers. Moreover high volume acquisition speed of our system allows imaging and later visualization of 3D retinal structures. The results of imaging healthy normal subjects as well as selected patients will be presented.
The combination of AO and video–rate Fourier–domain OCT offers the potential to increase sensitivity and transverse resolution as compared to imaging with OCT alone. High acquisition speed combined with improved transverse resolution allows insight into the 3D microscopic structure of the living retina hitherto possible only with histological samples.
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